TY - JOUR
T1 - Triculamin
T2 - An Unusual Lasso Peptide with Potent Antimycobacterial Activity
AU - Andersen, Frederikke D.
AU - Pedersen, Katja D.
AU - Juhl, Dennis Wilkens
AU - Mygind, Tobias
AU - Chopin, Paul
AU - B. Svenningsen, Esben
AU - Poulsen, Thomas B.
AU - Braad Lund, Marie
AU - Schramm, Andreas
AU - Gotfredsen, Charlotte H.
AU - Tørring, Thomas
PY - 2022
Y1 - 2022
N2 - Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) produced by microorganisms. Here we show that the two natural products triculamin and alboverticillin, originally isolated in 1967 and 1958, respectively, with potent and specific activity against mycobacteria are in fact the same lasso peptide. We solved the structure using 2D NMR spectroscopy and expanded on the previously reported bioactivity. Through genome sequencing, we identify the responsible biosynthetic gene clusters, which curiously revealed that, unlike any known lasso peptides, their precursor peptides appear to have a follower instead of a leader peptide.
AB - Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) produced by microorganisms. Here we show that the two natural products triculamin and alboverticillin, originally isolated in 1967 and 1958, respectively, with potent and specific activity against mycobacteria are in fact the same lasso peptide. We solved the structure using 2D NMR spectroscopy and expanded on the previously reported bioactivity. Through genome sequencing, we identify the responsible biosynthetic gene clusters, which curiously revealed that, unlike any known lasso peptides, their precursor peptides appear to have a follower instead of a leader peptide.
U2 - 10.1021/acs.jnatprod.2c00065
DO - 10.1021/acs.jnatprod.2c00065
M3 - Journal article
C2 - 35748039
SN - 0163-3864
VL - 85
SP - 1514
EP - 1521
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 6
ER -