Triculamin: An Unusual Lasso Peptide with Potent Antimycobacterial Activity

Frederikke D. Andersen, Katja D. Pedersen, Dennis Wilkens Juhl, Tobias Mygind, Paul Chopin, Esben B. Svenningsen, Thomas B. Poulsen, Marie Braad Lund, Andreas Schramm, Charlotte H. Gotfredsen, Thomas Tørring*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) produced by microorganisms. Here we show that the two natural products triculamin and alboverticillin, originally isolated in 1967 and 1958, respectively, with potent and specific activity against mycobacteria are in fact the same lasso peptide. We solved the structure using 2D NMR spectroscopy and expanded on the previously reported bioactivity. Through genome sequencing, we identify the responsible biosynthetic gene clusters, which curiously revealed that, unlike any known lasso peptides, their precursor peptides appear to have a follower instead of a leader peptide.
Original languageEnglish
JournalJournal of Natural Products
Number of pages8
ISSN0163-3864
DOIs
Publication statusAccepted/In press - 2022

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