Trends and approaches in N-Glycosylation engineering in Chinese hamster ovary cell culture

Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen

Research output: Contribution to conferencePosterResearchpeer-review

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Abstract

Chinese hamster ovary (CHO) cells have become the preferred expression system for the production of complex recombinantglycoproteins. It has been historically successful in industrial scale-up application and in generating human-like protein glycosylation.N-glycosylation of recombinant proteins, in particular, of those as drug substances, is extremely concerned in drug development andapproval, as it will largely affect their stability, efficacy, clearance rate and immunogenicity. Therefore to engineering N-glycosylationof CHO cell-derived recombinant proteins are extremely important. Here, we will summarize a group of recent strategies andapproaches and come up with case studies for N-glycosylation engineering in CHO cells and show several examples of relevantstudy cases from our research: 1) media and feed design, 2) culture process optimization, 3) substrate addition, 4) geneticengineering, 5) omics-based characterization, 6) mathematical modelling.
Original languageEnglish
Publication date2016
Number of pages1
Publication statusPublished - 2016
Event11th Danish Conference on Biotechnology and Molecular Biology - Hotel Munkebjerg, Vejle, Denmark
Duration: 26 May 201627 May 2016

Conference

Conference11th Danish Conference on Biotechnology and Molecular Biology
LocationHotel Munkebjerg
CountryDenmark
CityVejle
Period26/05/201627/05/2016

Bibliographical note

The poster won the first prize of "The best poster prizes".

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