Chinese hamster ovary (CHO) cells have become the preferred expression system for the production of complex recombinantglycoproteins. It has been historically successful in industrial scale-up application and in generating human-like protein glycosylation.N-glycosylation of recombinant proteins, in particular, of those as drug substances, is extremely concerned in drug development andapproval, as it will largely affect their stability, efficacy, clearance rate and immunogenicity. Therefore to engineering N-glycosylationof CHO cell-derived recombinant proteins are extremely important. Here, we will summarize a group of recent strategies andapproaches and come up with case studies for N-glycosylation engineering in CHO cells and show several examples of relevantstudy cases from our research: 1) media and feed design, 2) culture process optimization, 3) substrate addition, 4) geneticengineering, 5) omics-based characterization, 6) mathematical modelling.
|Number of pages||1|
|Publication status||Published - 2016|
|Event||11th Danish Conference on Biotechnology and Molecular Biology - Hotel Munkebjerg, Vejle, Denmark|
Duration: 26 May 2016 → 27 May 2016
|Conference||11th Danish Conference on Biotechnology and Molecular Biology|
|Period||26/05/2016 → 27/05/2016|