Trends and approaches in N-Glycosylation engineering in Chinese hamster ovary cell culture

Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen

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Abstract

Chinese hamster ovary (CHO) cells have become the preferred expression system for the production of complex recombinantglycoproteins. It has been historically successful in industrial scale-up application and in generating human-like protein glycosylation.N-glycosylation of recombinant proteins, in particular, of those as drug substances, is extremely concerned in drug development andapproval, as it will largely affect their stability, efficacy, clearance rate and immunogenicity. Therefore to engineering N-glycosylationof CHO cell-derived recombinant proteins are extremely important. Here, we will summarize a group of recent strategies andapproaches and come up with case studies for N-glycosylation engineering in CHO cells and show several examples of relevantstudy cases from our research: 1) media and feed design, 2) culture process optimization, 3) substrate addition, 4) geneticengineering, 5) omics-based characterization, 6) mathematical modelling.
Original languageEnglish
Publication date2016
Number of pages1
Publication statusPublished - 2016
EventPEGS Europe: Protein & Antibody Engineering Summit 2016 - Lisbon, Portugal
Duration: 31 Oct 20164 Nov 2016

Conference

ConferencePEGS Europe: Protein & Antibody Engineering Summit 2016
CountryPortugal
CityLisbon
Period31/10/201604/11/2016

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