Abstract
Original language | English |
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Article number | 203 |
Journal | Communications Biology |
Volume | 2 |
Number of pages | 13 |
DOIs | |
Publication status | Published - 2019 |
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TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells are enriched in the airways in human tuberculosis. / Wong, Emily B.; Gold, Marielle C.; Meermeier, Erin W.; Xulu, Bongiwe Z.; Khuzwayo, Sharon; Sullivan, Zuri A.; Mahyari, Eisa; Rogers, Zoe; Kløverpris, Hénrik; Sharma, Prabhat K.; Worley, Aneta H.; Lalloo, Umesh; Baijnath, Prinita; Ambaram, Anish; Naidoo, Leon; Suleman, Moosa; Madansein, Rajhmun; McLaren, James E.; Ladell, Kristin; Miners, Kelly L.; Price, David A.; Behar, Samuel M.; Nielsen, Morten; Kasprowicz, Victoria O.; Leslie, Alasdair; Bishai, William R.; Ndung'u, Thumbi; Lewinsohn, David M.
In: Communications Biology, Vol. 2, 203, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells are enriched in the airways in human tuberculosis
AU - Wong, Emily B.
AU - Gold, Marielle C.
AU - Meermeier, Erin W.
AU - Xulu, Bongiwe Z.
AU - Khuzwayo, Sharon
AU - Sullivan, Zuri A.
AU - Mahyari, Eisa
AU - Rogers, Zoe
AU - Kløverpris, Hénrik
AU - Sharma, Prabhat K.
AU - Worley, Aneta H.
AU - Lalloo, Umesh
AU - Baijnath, Prinita
AU - Ambaram, Anish
AU - Naidoo, Leon
AU - Suleman, Moosa
AU - Madansein, Rajhmun
AU - McLaren, James E.
AU - Ladell, Kristin
AU - Miners, Kelly L.
AU - Price, David A.
AU - Behar, Samuel M.
AU - Nielsen, Morten
AU - Kasprowicz, Victoria O.
AU - Leslie, Alasdair
AU - Bishai, William R.
AU - Ndung'u, Thumbi
AU - Lewinsohn, David M.
PY - 2019
Y1 - 2019
N2 - Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2+ semi-invariant TCRα that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models. Here, we report that a population of pro-inflammatory TRAV1-2+ CD8+ T cells are present in the airways and lungs of healthy individuals and are enriched in bronchoalveolar fluid of patients with active pulmonary tuberculosis (TB). High-throughput T cell receptor analysis reveals oligoclonal expansions of canonical and donor-unique TRAV1-2+ MAIT-consistent TCRα sequences within this population. Some of these cells demonstrate MR1-restricted mycobacterial reactivity and phenotypes suggestive of MAIT cell identity. These findings demonstrate enrichment of TRAV1-2+ CD8+ T cells with MAIT or MAIT-like features in the airways during active TB and suggest a role for these cells in the human pulmonary immune response to Mycobacterium tuberculosis.
AB - Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2+ semi-invariant TCRα that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models. Here, we report that a population of pro-inflammatory TRAV1-2+ CD8+ T cells are present in the airways and lungs of healthy individuals and are enriched in bronchoalveolar fluid of patients with active pulmonary tuberculosis (TB). High-throughput T cell receptor analysis reveals oligoclonal expansions of canonical and donor-unique TRAV1-2+ MAIT-consistent TCRα sequences within this population. Some of these cells demonstrate MR1-restricted mycobacterial reactivity and phenotypes suggestive of MAIT cell identity. These findings demonstrate enrichment of TRAV1-2+ CD8+ T cells with MAIT or MAIT-like features in the airways during active TB and suggest a role for these cells in the human pulmonary immune response to Mycobacterium tuberculosis.
U2 - 10.1038/s42003-019-0442-2
DO - 10.1038/s42003-019-0442-2
M3 - Journal article
VL - 2
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
M1 - 203
ER -