Transplanting Two Unique β-Glucanase catalytic Activities into one Multienzyme, Which Forms Glucose

Ole Olsen, Karl Kristian Thomsen, Jürgen Weber, Jens Øllgaard Duus, Ib Svendsen, Christina Wegener, Diter von Wettstein

Research output: Contribution to journalJournal articleResearchpeer-review

26 Downloads (Pure)

Abstract

Endo cellulases of plant pathogenic erwinias degrade cellulose as well as the cellulosic domains of barley (1-3,1-4)-β-glucan. Depolymerization of the latter substrate is mainly caused by (1-3,1-4)-β-glucanases, which hydrolyze (1-4)-β glycosidic linkages adjacent to (1-3)-β linkages. To construct an enzyme for efficient degradation of barley (1-3,1-4)-β-glucan, the sequence encoding the catalytic domain and interdomain linker of the cellulase from Erwinia carotovora subspecies atroseptica was fused to that for the heat stable Bacillus hybrid, H(A12-M)ΔY13 (1-3,1-4)-β-glucanase. The chimeric enzyme secreted from Escherichia coil cells did not remain covalently assembled as judged by SDS-PAGE. However, the glycosylated and intact enzyme (denoted CELGLU) is secreted from the yeast Pichia pastoris. CELGLU exhibits both cellulase and (1-3,1-4)-β-glucanase catalytic activities, and was accordingly classified a true multienzyme. HPLC and NMR analyses revealed that among the products from CELGLU, di- and trimeric oligosaccharides were identical to those produced by the parental cellulase. Tetrameric oligosaccharides, derived from the (1-3,1-4)-β-glucanase activity of CELGLU, were further degraded by the cellulase moiety to yield glucose and trimers. Compared with the parental enzymes, CELGLU exhibits substantially higher V(max) for degradation of both soluble cellulose and barley (1-3, 1-4)-β- glucan. These findings point to construction of multienzymes as an effective approach for engineering enzymes with novel characteristics.
Original languageEnglish
JournalBIO-TECHNOLOGY
Volume14
Pages (from-to)71-76
ISSN0733-222X
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Dive into the research topics of 'Transplanting Two Unique β-Glucanase catalytic Activities into one Multienzyme, Which Forms Glucose'. Together they form a unique fingerprint.

Cite this