Translocation of flexible polymersomes across pores at the nanoscale

Carla Pegoraro, Denis Cecchin, Peter Jeppe Madsen, Nicholas J. Warren, Steven P. Armes, Sheila MacNeil, Andrew Lewis, Giuseppe Battaglia*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Hierarchical biological systems such as tissues and organs are often characterised by highly crowded and packed environments with nanoscopic interconnections between them. Engineering nanovectors that can penetrate and diffuse across these is critical to ensure enhanced delivery and targeting. Here we demonstrate that flexible polymeric vesicles, known as polymersomes, enable the translocation of large macromolecules across both synthetic and biological porous systems. We compare the translocation across narrow pores of different polymersome formulations. We demonstrate that effective translocation depends on the right combination of mechanical properties and surface lubrication. We prove that with the effect of external gradients (e.g. osmotic pressure, capillarity, hydration, etc.) polymersomes can translocate across pores with diameters one order of magnitude smaller without breaking. We demonstrate that these properties are essential to develop effective tissue penetration and show polymersome mediated transdermal delivery of large macromolecules such as dextran and antibodies using human ex vivo skin. This journal is

Original languageEnglish
JournalBiomaterials Science
Volume2
Issue number5
Pages (from-to)680-692
ISSN2047-4830
DOIs
Publication statusPublished - 2014
Externally publishedYes

Cite this

Pegoraro, C., Cecchin, D., Jeppe Madsen, P., Warren, N. J., Armes, S. P., MacNeil, S., Lewis, A., & Battaglia, G. (2014). Translocation of flexible polymersomes across pores at the nanoscale. Biomaterials Science, 2(5), 680-692. https://doi.org/10.1039/c3bm60294j