Translating silicon nanowire BioFET sensor-technology to embedded point-of-care medical diagnostics

Andrea Pfreundt, Azeem Zulfiqar, François Patou, Lars Andresen, Maria Dimaki, Winnie Edith Svendsen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearch

    Abstract

    Silicon nanowire and nanoribbon biosensors have shown great promise in the detection of biomarkers at very low concentrations. Their high sensitivity makes them ideal candidates for use in early-stage medical diagnostics and further disease monitoring where low amounts of biomarkers need to be detected. However, in order to translate this technology from the bench to the bedside, a number of key issues need to be taken into consideration: Integrating nanobiosensors-based technology requires to overcome the difficult tradeoff between imperatives for high device reproducibilty and associated rising fabrication costs. Also the translation of nano-scale sensor technology into daily-use point-of-care devices requires acknowledgement of the end-user requirements, making device portability and human-interfacing a focus point in device development. Sample handling or purification for instance, should be addressed in an automated way.
    Here, we are presenting the concept of a polysilicon nanoribbon sensor array integrated with multiplexed microfluidic functionalization, automated calibration and sample handling for flexible diagnostics from finger prick blood samples.
    Functionalization of the sensor surface is performed in a controlled microfluidic environment and can be monitored in real-time to ensure reproducible results. In a simple temporary PDMS device, multiple parallel pathways enable straight-forward selective functionalization for different biomarkers. Common diagnostic essays, which require a specific set of biomakers to be identified and quantified simultaneously, can thus be readily translated onto this platform. After hydrogen termination of the silicon surface an alkyne monolayer is formed based on a hydrosilylation process. Antibodies and other receptor proteins can then be immobilized in a parallel manner without the use of a spotting system using various chemistries depending on the chosen headgroup in the monolayer. The system is designed to work with a single tube at the outlet and is able to mix and deliver immobilization reactants and antibody solution as well as washing buffer to the sensor surface.
    Original languageEnglish
    Title of host publicationProceedings of The Gordon Research Conference on the Physics and Chemistry of Microfluidics
    Publication date2013
    Publication statusPublished - 2013
    EventThe Gordon Research Conference on the Physics and Chemistry of Microfluidics: Challenges, Advances and New Technologies for Diagnostics - Renaissance Tuscany Il Ciocco Resort, Lucca, Italy
    Duration: 9 Jun 201314 Jun 2013

    Conference

    ConferenceThe Gordon Research Conference on the Physics and Chemistry of Microfluidics
    LocationRenaissance Tuscany Il Ciocco Resort
    CountryItaly
    CityLucca
    Period09/06/201314/06/2013

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