Transforming Escherichia coli Proteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis

Alexander Klaus Mengele, Dominik Weixler, Sebastian Amthor, Bernhard J. Eikmanns, Gerd M. Seibold, Sven Rau*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

During the light-dependent reaction of photosynthesis green plants couple photoinduced cascades of redox reactions with transmembrane proton translocations to generate reducing equivalents and chemical energy in the form of NADPH (nicotinamide adenine dinucleotide phosphate) and ATP (adenosine triphosphate), respectively. We mimic these basic processes by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli. Upon irradiation with visible light, the interplay of photocatalytic nicotinamide reduction and enzymatic membrane-located respiration leads to the simultaneous formation of two biologically active cofactors, NADH (nicotinamide adenine dinucleotide) and ATP, respectively. This inorganic-biologic hybrid system thus emulates the cofactor delivering function of an active chloroplast.
Original languageEnglish
Article numbere202114842
JournalAngewandte Chemie International Edition
Volume61
Issue number11
Number of pages8
ISSN1433-7851
DOIs
Publication statusPublished - 2022

Keywords

  • Biocatalysis
  • Cofactors
  • Photocatalysis
  • Photosynthesis
  • Synthetic biology

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