Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events

Mogens Nicolaisen; Rolf Geisen

doi:10.1016/S0944-5013(96)80025-5

Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events

Mogens Nicolaisen, Rolf Geisen

Institute for Hygiene and Toxicology

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per μg DNA per 1 x 107 protoplasts. Southern blotting revealed different types of integration into the genome of P. freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more then 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR.

Original language	English
Journal	Microbiological Research
Volume	151
Issue number	3
Pages (from-to)	281-284
ISSN	0944-5013
DOIs	https://doi.org/10.1016/S0944-5013(96)80025-5
Publication status	Published - 1996

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title = "Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events",

abstract = "A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per μg DNA per 1 x 107 protoplasts. Southern blotting revealed different types of integration into the genome of P. freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more then 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR.",

author = "Mogens Nicolaisen and Rolf Geisen",

year = "1996",

doi = "10.1016/S0944-5013(96)80025-5",

language = "English",

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pages = "281--284",

journal = "Microbiological Research",

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T1 - Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events

AU - Nicolaisen, Mogens

AU - Geisen, Rolf

PY - 1996

Y1 - 1996

N2 - A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per μg DNA per 1 x 107 protoplasts. Southern blotting revealed different types of integration into the genome of P. freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more then 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR.

AB - A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per μg DNA per 1 x 107 protoplasts. Southern blotting revealed different types of integration into the genome of P. freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more then 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR.

U2 - 10.1016/S0944-5013(96)80025-5

DO - 10.1016/S0944-5013(96)80025-5

M3 - Journal article

SN - 0944-5013

VL - 151

SP - 281

EP - 284

JO - Microbiological Research

JF - Microbiological Research

IS - 3

ER -

Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events

Abstract

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