Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

A.T. Trieu, S.H. Burleigh, I.V. Kardailsky, I.E. Maldonado-Mendoza, W.K. Versaw, L.A. Blaylock, H. Shin, T.-J. Chiou, H. Katagi, G.R. Dewbre, D. Weigel, M.J. Harrison

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    Abstract

    Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.
    Original languageEnglish
    JournalPlant Journal
    Volume22
    Issue number6
    Pages (from-to)531-541
    ISSN0960-7412
    DOIs
    Publication statusPublished - 2000

    Cite this

    Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado-Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T-J., Katagi, H., Dewbre, G. R., Weigel, D., & Harrison, M. J. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant Journal, 22(6), 531-541. https://doi.org/10.1046/j.1365-313x.2000.00757.x