Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

A.T. Trieu; S.H. Burleigh; I.V. Kardailsky; I.E. Maldonado-Mendoza; W.K. Versaw; L.A. Blaylock; H. Shin; T.-J. Chiou; H. Katagi; G.R. Dewbre; D. Weigel; M.J. Harrison

doi:10.1046/j.1365-313x.2000.00757.x

Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

A.T. Trieu, S.H. Burleigh, I.V. Kardailsky, I.E. Maldonado-Mendoza, W.K. Versaw, L.A. Blaylock, H. Shin, T.-J. Chiou, H. Katagi, G.R. Dewbre, D. Weigel, M.J. Harrison

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

Original language	English
Journal	Plant Journal
Volume	22
Issue number	6
Pages (from-to)	531-541
ISSN	0960-7412
DOIs	https://doi.org/10.1046/j.1365-313x.2000.00757.x
Publication status	Published - 2000

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Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado-Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T.-J., Katagi, H., Dewbre, G. R., Weigel, D., & Harrison, M. J. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant Journal, 22(6), 531-541. https://doi.org/10.1046/j.1365-313x.2000.00757.x

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title = "Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium",

abstract = "Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes. ",

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doi = "10.1046/j.1365-313x.2000.00757.x",

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T1 - Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

AU - Trieu, A.T.

AU - Burleigh, S.H.

AU - Kardailsky, I.V.

AU - Maldonado-Mendoza, I.E.

AU - Versaw, W.K.

AU - Blaylock, L.A.

AU - Shin, H.

AU - Chiou, T.-J.

AU - Katagi, H.

AU - Dewbre, G.R.

AU - Weigel, D.

AU - Harrison, M.J.

PY - 2000

Y1 - 2000

N2 - Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

AB - Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

KW - Planteproduktion og stofomsætning

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DO - 10.1046/j.1365-313x.2000.00757.x

M3 - Journal article

SN - 0960-7412

VL - 22

SP - 531

EP - 541

JO - Plant Journal

JF - Plant Journal

IS - 6

ER -

Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

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