Transcriptomic variation in a coral reveals pathways of clonal organisation

Line K Bay, Henrik Bjørn Nielsen, Hanne Østergaard Jarmer, Francois Seneca, Madeleine J H van Oppe

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A microarray study was undertaken to examine the potential for clonal gene expression variation in a branching reef building coral, Acropora millepora. The role of small-scale gradients in light and water flow was examined by comparing gene expression levels between branch elevation (tip and base) and position (centre and edge) of replicate coral colonies (n=3). Analyses of variance revealed that almost 60% of variation in gene expression was present between colonies and 34 genes were considered differentially expressed between colonies (minimum P=6.5 x 10(-4)). These genes are associated with energy metabolism, protein biosynthesis and cell-cell recognition representing either genotypic variation in gene expression or the effects of specific environmental conditions that affect patterns of energy acquisition, growth and pathogen encounters. Less variation was present between central and peripheral branches (7%) and only a single gene was deemed differentially expressed (P=1.493 x 10(-3)). The function of this gene, a phosphatidylserine decarboxylase, suggests different growth patterns between branch positions within colonies and is consistent with the usual higher growth rates on the perimeter of corymbose-like branching coral colonies such as A. millepora. Four genes were differentially expressed between the tip and base of branches (P=3.239 x 10(-4)) and were associated with lysosome lipase activity and fluorescence, suggesting that branch tips may encounter higher pathogen loads or levels of mechanical stress and require greater levels of photo-protection associated with higher water flow and light levels. This study therefore confirms transcriptomic variation in response to small-scale environmental gradients consistent with differential resource allocation in clonal coral colonies. (C) 2009 Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalMarine Genomics
    Volume2
    Issue number2
    Pages (from-to)119-125
    ISSN1874-7787
    DOIs
    Publication statusPublished - 2009

    Cite this

    K Bay, L., Nielsen, H. B., Jarmer, H. Ø., Seneca, F., & J H van Oppe, M. (2009). Transcriptomic variation in a coral reveals pathways of clonal organisation. Marine Genomics, 2(2), 119-125. https://doi.org/10.1016/j.margen.2009.07.004
    K Bay, Line ; Nielsen, Henrik Bjørn ; Jarmer, Hanne Østergaard ; Seneca, Francois ; J H van Oppe, Madeleine. / Transcriptomic variation in a coral reveals pathways of clonal organisation. In: Marine Genomics. 2009 ; Vol. 2, No. 2. pp. 119-125.
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    title = "Transcriptomic variation in a coral reveals pathways of clonal organisation",
    abstract = "A microarray study was undertaken to examine the potential for clonal gene expression variation in a branching reef building coral, Acropora millepora. The role of small-scale gradients in light and water flow was examined by comparing gene expression levels between branch elevation (tip and base) and position (centre and edge) of replicate coral colonies (n=3). Analyses of variance revealed that almost 60{\%} of variation in gene expression was present between colonies and 34 genes were considered differentially expressed between colonies (minimum P=6.5 x 10(-4)). These genes are associated with energy metabolism, protein biosynthesis and cell-cell recognition representing either genotypic variation in gene expression or the effects of specific environmental conditions that affect patterns of energy acquisition, growth and pathogen encounters. Less variation was present between central and peripheral branches (7{\%}) and only a single gene was deemed differentially expressed (P=1.493 x 10(-3)). The function of this gene, a phosphatidylserine decarboxylase, suggests different growth patterns between branch positions within colonies and is consistent with the usual higher growth rates on the perimeter of corymbose-like branching coral colonies such as A. millepora. Four genes were differentially expressed between the tip and base of branches (P=3.239 x 10(-4)) and were associated with lysosome lipase activity and fluorescence, suggesting that branch tips may encounter higher pathogen loads or levels of mechanical stress and require greater levels of photo-protection associated with higher water flow and light levels. This study therefore confirms transcriptomic variation in response to small-scale environmental gradients consistent with differential resource allocation in clonal coral colonies. (C) 2009 Elsevier B.V. All rights reserved.",
    author = "{K Bay}, Line and Nielsen, {Henrik Bj{\o}rn} and Jarmer, {Hanne {\O}stergaard} and Francois Seneca and {J H van Oppe}, Madeleine",
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    K Bay, L, Nielsen, HB, Jarmer, HØ, Seneca, F & J H van Oppe, M 2009, 'Transcriptomic variation in a coral reveals pathways of clonal organisation', Marine Genomics, vol. 2, no. 2, pp. 119-125. https://doi.org/10.1016/j.margen.2009.07.004

    Transcriptomic variation in a coral reveals pathways of clonal organisation. / K Bay, Line; Nielsen, Henrik Bjørn; Jarmer, Hanne Østergaard; Seneca, Francois; J H van Oppe, Madeleine.

    In: Marine Genomics, Vol. 2, No. 2, 2009, p. 119-125.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Transcriptomic variation in a coral reveals pathways of clonal organisation

    AU - K Bay, Line

    AU - Nielsen, Henrik Bjørn

    AU - Jarmer, Hanne Østergaard

    AU - Seneca, Francois

    AU - J H van Oppe, Madeleine

    PY - 2009

    Y1 - 2009

    N2 - A microarray study was undertaken to examine the potential for clonal gene expression variation in a branching reef building coral, Acropora millepora. The role of small-scale gradients in light and water flow was examined by comparing gene expression levels between branch elevation (tip and base) and position (centre and edge) of replicate coral colonies (n=3). Analyses of variance revealed that almost 60% of variation in gene expression was present between colonies and 34 genes were considered differentially expressed between colonies (minimum P=6.5 x 10(-4)). These genes are associated with energy metabolism, protein biosynthesis and cell-cell recognition representing either genotypic variation in gene expression or the effects of specific environmental conditions that affect patterns of energy acquisition, growth and pathogen encounters. Less variation was present between central and peripheral branches (7%) and only a single gene was deemed differentially expressed (P=1.493 x 10(-3)). The function of this gene, a phosphatidylserine decarboxylase, suggests different growth patterns between branch positions within colonies and is consistent with the usual higher growth rates on the perimeter of corymbose-like branching coral colonies such as A. millepora. Four genes were differentially expressed between the tip and base of branches (P=3.239 x 10(-4)) and were associated with lysosome lipase activity and fluorescence, suggesting that branch tips may encounter higher pathogen loads or levels of mechanical stress and require greater levels of photo-protection associated with higher water flow and light levels. This study therefore confirms transcriptomic variation in response to small-scale environmental gradients consistent with differential resource allocation in clonal coral colonies. (C) 2009 Elsevier B.V. All rights reserved.

    AB - A microarray study was undertaken to examine the potential for clonal gene expression variation in a branching reef building coral, Acropora millepora. The role of small-scale gradients in light and water flow was examined by comparing gene expression levels between branch elevation (tip and base) and position (centre and edge) of replicate coral colonies (n=3). Analyses of variance revealed that almost 60% of variation in gene expression was present between colonies and 34 genes were considered differentially expressed between colonies (minimum P=6.5 x 10(-4)). These genes are associated with energy metabolism, protein biosynthesis and cell-cell recognition representing either genotypic variation in gene expression or the effects of specific environmental conditions that affect patterns of energy acquisition, growth and pathogen encounters. Less variation was present between central and peripheral branches (7%) and only a single gene was deemed differentially expressed (P=1.493 x 10(-3)). The function of this gene, a phosphatidylserine decarboxylase, suggests different growth patterns between branch positions within colonies and is consistent with the usual higher growth rates on the perimeter of corymbose-like branching coral colonies such as A. millepora. Four genes were differentially expressed between the tip and base of branches (P=3.239 x 10(-4)) and were associated with lysosome lipase activity and fluorescence, suggesting that branch tips may encounter higher pathogen loads or levels of mechanical stress and require greater levels of photo-protection associated with higher water flow and light levels. This study therefore confirms transcriptomic variation in response to small-scale environmental gradients consistent with differential resource allocation in clonal coral colonies. (C) 2009 Elsevier B.V. All rights reserved.

    U2 - 10.1016/j.margen.2009.07.004

    DO - 10.1016/j.margen.2009.07.004

    M3 - Journal article

    VL - 2

    SP - 119

    EP - 125

    JO - Marine Genomics

    JF - Marine Genomics

    SN - 1874-7787

    IS - 2

    ER -