Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions

Hanne Østergaard Jarmer, R. Berka, Steen Knudsen, Hans Henrik Saxild

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    DNA microarrays were used to analyze the changes in gene expression in Bacillus subtilis strain 168 when nitrogen limiting (glutamate) and nitrogen excess (ammonium plus glutamate) growth conditions were compared. Among more than 100 genes that were significantly induced during nitrogen starvation we detected the comG, comF, comE, nin-nucA and comK transcription units together with recA. DNA was added to B. subtilis grown in minimal medium with glutamate as the sole nitrogen source and it was demonstrated that the cells were competent. Based on these observations we propose a simplification of previously designed one-step transformation procedures for B. subtilis strain 168.
    Original languageEnglish
    JournalFEMS Microbiology Letters
    Volume206
    Issue number2
    Pages (from-to)197-200
    ISSN0378-1097
    Publication statusPublished - 2002

    Fingerprint Dive into the research topics of 'Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions'. Together they form a unique fingerprint.

    Cite this