Abstract
Out of 8500 genes, FVIIa stimulation induced differential regulation of 39 genes most of which were not previously recognized as FVIIa regulated. All genes regulated by FVIIa were similarly regulated by a PAR2 agonist peptide confirming FVIIa signaling via PAR2. An appreciable fraction of the PAR2-regulated genes was also regulated by a PAR1 agonist peptide suggesting extensive redundancy between FVIIa/PAR2 signaling and thrombin/PAR1 signaling. The FVIIa regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell line.
| Original language | English |
|---|---|
| Journal | Journal of Thrombosis and Haemostasis |
| Volume | 5 |
| Issue number | 8 |
| Pages (from-to) | 1588-1597 |
| ISSN | 1538-7933 |
| Publication status | Published - 2007 |
Keywords
- Binding Sites
- Cell Line, Tumor
- Factor VIIa
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Neoplasms
- Oligonucleotide Array Sequence Analysis
- Peptides
- Receptor, PAR-1
- Receptor, PAR-2
- Signal Transduction
- Thromboplastin
- Transcription, Genetic
- 9035-58-9 Thromboplastin
- EC 3.4.21.21 Factor VIIa