Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells.

T Albrektsen, B B Sørensen, G M Hjortø, Jan Fleckner, L V M Rao, L C Petersen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Out of 8500 genes, FVIIa stimulation induced differential regulation of 39 genes most of which were not previously recognized as FVIIa regulated. All genes regulated by FVIIa were similarly regulated by a PAR2 agonist peptide confirming FVIIa signaling via PAR2. An appreciable fraction of the PAR2-regulated genes was also regulated by a PAR1 agonist peptide suggesting extensive redundancy between FVIIa/PAR2 signaling and thrombin/PAR1 signaling. The FVIIa regulated genes encode cytokines, chemokines and growth factors, and the gene repertoire induced by FVIIa in MDA-MB-231 cells is consistent with a role for TF-FVIIa signaling in regulation of a wound healing type of response. Interestingly, a number of genes regulated exclusively by FVIIa/PAR2-mediated cell signaling in MDA-MB-231 cells were regulated by thrombin and a PAR1 agonist, but not by FVIIa, in the TF-expressing glioblastoma U373 cell line.
    Original languageEnglish
    JournalJournal of Thrombosis and Haemostasis
    Volume5
    Issue number8
    Pages (from-to)1588-1597
    ISSN1538-7933
    Publication statusPublished - 2007

    Keywords

    • Binding Sites
    • Cell Line, Tumor
    • Factor VIIa
    • Gene Expression Profiling
    • Gene Expression Regulation, Neoplastic
    • Humans
    • Neoplasms
    • Oligonucleotide Array Sequence Analysis
    • Peptides
    • Receptor, PAR-1
    • Receptor, PAR-2
    • Signal Transduction
    • Thromboplastin
    • Transcription, Genetic
    • 9035-58-9 Thromboplastin
    • EC 3.4.21.21 Factor VIIa

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