TRANS COMPLEMENTATION BY RNA OF DEFECTIVE FOOT-AND-MOUTH-DISEASE VIRUS INTERNAL RIBOSOME ENTRY SITE ELEMENTS

J. Drew, Graham Belsham

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A region of about 435 bases from the 5' noncoding region of foot-and-mouth disease virus RNA directs internal initiation of protein synthesis. This region, termed the internal ribosome entry site (IRES), is predicted to contain extensive secondary structure. Precise deletion of five predicted secondary structure features has been performed. The mutant IRES elements have been constructed into vectors which express bicistronic mRNAs and assayed within cells. Each of the modified IRES elements was defective in directing internal initiation when assayed alone. However, coexpression of an intact foot-and-mouth disease virus IRES complemented four of these defective elements to an efficiency of up to 80% of wild-type activity. No complementation was observed with the structurally analogous element from encephalomyocarditis virus. The role of RNA-RNA interactions in the function of the picornavirus IRES is discussed.
Original languageEnglish
JournalJournal of Virology
Volume68
Issue number2
Pages (from-to)697-703
ISSN0022-538X
Publication statusPublished - 1994
Externally publishedYes

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