Towards a portable microchip system with integrated thermal control and polymer waveguides for real-time PCR

Zhenyu Wang, Andrea Sekulovic, Jörg Peter Kutter, Dang Duong Bang, Anders Wolff

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A novel real-time PCR microchip platform with integrated thermal system and polymer waveguides has been developed. The integrated polymer optical system for real-time monitoring of PCR was fabricated in the same SU-8 layer as the PCR chamber, without additional masking steps. Two suitable DNA binding dyes, SYTOX Orange and TO-PRO-3, were selected and tested for the real-time PCR processes. As a model, cadF gene of Campylobacter jejuni has been amplified on the microchip. Using the integrated optical system of the real-time PCR microchip, the measured cycle threshold values of the real-time PCR performed with a dilution series of C. jejuni DNA template (2 to 200 pg/mu l) could be quantitatively detected and compared with a conventional post-PCR analysis (DNA gel electrophoresis). The presented approach provided reliable real-time quantitative information of the PCR amplification of the targeted gene. With the integrated optical system, the reaction dynamics at any location inside the micro reaction chamber can easily be monitored.
    Original languageEnglish
    JournalElectrophoresis
    Volume27
    Issue number24
    Pages (from-to)5051-5058
    ISSN0173-0835
    DOIs
    Publication statusPublished - 2006

    Keywords

    • polymer waveguides
    • SU-8
    • real-time PCR
    • integrated thermal system

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