Abstract
Biocatalysis has the potential to provide the chemical industry with several advantages for the production
of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately
limited due to the high costs related to the production of the biocatalyst. We have immobilized
Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst,
i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation
reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and
polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier
was significantly less than the cost of commercially available Novozym®435, indicating a potential
for decreased cost. Also the stability of Accurel MP1000 bound enzyme during repeated use matched that
of Novozym®435. Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent
stability in repeated batches at 70 ◦C.
Keyword: Esterification,Biocatalyst stability,Batch stirred tank reactor,Amidation,Packed-bed reactor
Keyword: Esterification,Biocatalyst stability,Batch stirred tank reactor,Amidation,Packed-bed reactor
Original language | English |
---|---|
Journal | Journal of Molecular Catalysis B: Enzymatic |
Volume | 68 |
Pages (from-to) | 200-205 |
ISSN | 1381-1177 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |