Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters: Validation in a multicenter collaborative trial

Peter Stephensen Lübeck, N. Cook, M. Wagner, P. Fach, Jeffrey Hoorfar

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C. coli, and C. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100%. The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume69
Issue number9
Pages (from-to)5670-5672
ISSN0099-2240
DOIs
Publication statusPublished - 2003

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