Topographic analysis of the toxic Gef protein from Escherichia coli

Annette Refn, Søren Molin, Poul Andersson

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The chromosomal gef gene of Escherichia coli is a member of the gef gene family which encodes strongly toxic proteins of about 50 amino acids. We demonstrate here that the Gef protein is detectable by anti-peptide antibodies. Furthermore, we show that Gef is anchored in the cytoplasmic membrane by the N-terminal part of the protein, and that the C-terminal part is localized in the periplasm in a dimeric form with at least one disulphide bond. By mutagenesis of gef it is shown that the periplasmic portion of Gef encodes the toxic domain and that the dimerization of Gef is not essential for the toxic effect.
Original languageEnglish
JournalMolecular Microbiology
Volume5
Issue number7
Pages (from-to)1627-1637
ISSN0950-382X
DOIs
Publication statusPublished - 1991
Externally publishedYes

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