TOL plasmid carriage enhances biofilm formation and increases extracellular DNA content in Pseudomonas putida KT2440

Paul D'Alvise, O.R. Sjoholm, T. Yankelevich, Y.J. Jin, S. Wuertz, Barth F. Smets

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Adherent growth of Pseudomonas putida KT2440 with and without the TOL plasmid (pWWO) at the solid-liquid and air-liquid interface was examined. We compared biofilm formation on glass in flow cells, and assayed pellicle (air-liquid interface biofilm) formation in stagnant liquid cultures by confocal laser scanning microscopy. The TOL-carrying strains formed pellicles and thick biofilms, whereas the same strains without the plasmid displayed little adherent growth. Microscopy using fluorescent nucleic acid-specific stains revealed differences in the production of extracellular polymeric substances: TOL carriage leads to more extracellular DNA (eDNA) in pellicles and biofilms. Pellicles were dissolved by DNase I treatment. Enhanced cell lysis due to plasmid carriage was ruled out as the mechanism for eDNA release. We report, for the first time, that carriage of a conjugative plasmid leads to increased biofilm formation by production of eDNA.
Original languageEnglish
JournalF E M S Microbiology Letters
Volume312
Issue number1
Pages (from-to)84-92
ISSN0378-1097
DOIs
Publication statusPublished - 2010

Keywords

  • biofilm
  • TOL plasmid
  • extracellular DNA
  • air-liquid interface

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