Tissue-specific distribution patterns of retinoids and didehydroretinoids in rainbow trout Oncorhynchus mykiss

Manuel Gesto, L. Filipe C. Castro, Maria Armanda Reis-Henriques, Miguel Machado Santos

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Retinoids (vitamin A) are known to be involved in many key biological functions in mammals, such as embryonic development, reproduction or vision. Besides standard vitamin A forms, freshwater fish tissues contain high levels of didehydroretinoids or vitamin A(2) forms. However, the tissue distribution, metabolism and function of both standard and particularly the didehydroretinoids are still poorly known in fish. In this study, we have quantified the levels of retinoids, including retinol, retinaldehyde, retinyl palmitate and their corresponding didehydro forms, as well as the levels of the active polar retinoids all-trans-, 9-cis- and 13-cis-retinoic acid in distinct tissues of juvenile rainbow trout. Our results indicate that the liver is clearly the main retinoid storage tissue in juvenile rainbow trout. Didehydroretinoids were dominant over retinoids in all analyzed tissues with the exception of plasma. Additionally, significant differences among tissues were observed between retinoids and didehydroretinoids, such as differences in the ester profiles and the proportions between free and esterified forms, suggesting that mechanisms that favor the utilization or storage of one of the other groups of compounds might exist in fish. Our data also show the presence of polar retinoids in different tissues of fish at the fmol/g scale. Overall, this study clearly demonstrates the presence of tissue-specific patterns of accumulation of both polar and nonpolar retinoids in fish tissues. The biological relevance of these findings should be the focus of future studies. (C) 2011 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology
Volume161
Issue number1
Pages (from-to)69-78
ISSN1096-4959
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • BIOCHEMISTRY
  • ZOOLOGY
  • PERFORMANCE LIQUID-CHROMATOGRAPHY
  • SALMON SALMO-SALAR
  • VITAMIN-A
  • MASS-SPECTROMETRY
  • STELLATE CELLS
  • ENDOGENOUS RETINOIDS
  • BIOLOGICAL SAMPLES
  • HYPERVITAMINOSIS-A
  • RETINYL ESTERS
  • XENOPUS-LAEVIS
  • Retinoid
  • Trout
  • HPLC
  • Didehydroretinoid
  • Vitamin A

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