Tissue-based map of the human proteome

Mathias Uhlén; Linn Fagerberg; Björn M. Hallström; Cecilia Lindskog; Per Oksvold; Adil Mardinoglu; Åsa Sivertsson; Caroline Kampf; Evelina Sjöstedt; Anna Asplund; IngMarie Olsson; Karolina Edlund; Emma Lundberg; Sanjay Navani; Cristina Al-Khalili Szigyarto; Jacob Odeberg; Dijana Djureinovic; Jenny Ottosson Takanen; Sophia Hober; Tove Alm; Per-Henrik Edqvist; Holger Berling; Hanna Tegel; Jan Mulder; Johan Rockberg; Peter Nilsson; Jochen M. Schwenk; Marica Hamsten; Kalle von Feilitzen; Mattias Forsberg; Lukas Persson; Fredric Johansson; Martin Zwahlen; Gunnar von Heijne; Jens Nielsen; Fredrik Pontén

doi:10.1126/science.1260419

Tissue-based map of the human proteome

Mathias Uhlén, Linn Fagerberg, Björn M. Hallström, Cecilia Lindskog, Per Oksvold, Adil Mardinoglu, Åsa Sivertsson, Caroline Kampf, Evelina Sjöstedt, Anna Asplund, IngMarie Olsson, Karolina Edlund, Emma Lundberg, Sanjay Navani, Cristina Al-Khalili Szigyarto, Jacob Odeberg, Dijana Djureinovic, Jenny Ottosson Takanen, Sophia Hober, Tove AlmPer-Henrik Edqvist, Holger Berling, Hanna Tegel, Jan Mulder, Johan Rockberg, Peter Nilsson, Jochen M. Schwenk, Marica Hamsten, Kalle von Feilitzen, Mattias Forsberg, Lukas Persson, Fredric Johansson, Martin Zwahlen, Gunnar von Heijne, Jens Nielsen, Fredrik Pontén

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.

Original language	English
Article number	1260419
Journal	Science (New York, N.Y.)
Volume	347
Issue number	6220
Pages (from-to)	394-
Number of pages	9
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.1260419
Publication status	Published - 2015

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Uhlén, M., Fagerberg, L., Hallström, B. M., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, Å., Kampf, C., Sjöstedt, E., Asplund, A., Olsson, I., Edlund, K., Lundberg, E., Navani, S., Szigyarto, C. A-K., Odeberg, J., Djureinovic, D., Takanen, J. O., Hober, S., ... Pontén, F. (2015). Tissue-based map of the human proteome. Science (New York, N.Y.), 347(6220), 394-. [1260419]. https://doi.org/10.1126/science.1260419

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abstract = "Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.",

author = "Mathias Uhl{\'e}n and Linn Fagerberg and Hallstr{\"o}m, {Bj{\"o}rn M.} and Cecilia Lindskog and Per Oksvold and Adil Mardinoglu and {\AA}sa Sivertsson and Caroline Kampf and Evelina Sj{\"o}stedt and Anna Asplund and IngMarie Olsson and Karolina Edlund and Emma Lundberg and Sanjay Navani and Szigyarto, {Cristina Al-Khalili} and Jacob Odeberg and Dijana Djureinovic and Takanen, {Jenny Ottosson} and Sophia Hober and Tove Alm and Per-Henrik Edqvist and Holger Berling and Hanna Tegel and Jan Mulder and Johan Rockberg and Peter Nilsson and Schwenk, {Jochen M.} and Marica Hamsten and {von Feilitzen}, Kalle and Mattias Forsberg and Lukas Persson and Fredric Johansson and Martin Zwahlen and {von Heijne}, Gunnar and Jens Nielsen and Fredrik Pont{\'e}n",

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doi = "10.1126/science.1260419",

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Uhlén, M, Fagerberg, L, Hallström, BM, Lindskog, C, Oksvold, P, Mardinoglu, A, Sivertsson, Å, Kampf, C, Sjöstedt, E, Asplund, A, Olsson, I, Edlund, K, Lundberg, E, Navani, S, Szigyarto, CA-K, Odeberg, J, Djureinovic, D, Takanen, JO, Hober, S, Alm, T, Edqvist, P-H, Berling, H, Tegel, H, Mulder, J, Rockberg, J, Nilsson, P, Schwenk, JM, Hamsten, M, von Feilitzen, K, Forsberg, M, Persson, L, Johansson, F, Zwahlen, M, von Heijne, G, Nielsen, J & Pontén, F 2015, 'Tissue-based map of the human proteome', Science (New York, N.Y.), vol. 347, no. 6220, 1260419, pp. 394-. https://doi.org/10.1126/science.1260419

TY - JOUR

T1 - Tissue-based map of the human proteome

AU - Uhlén, Mathias

AU - Fagerberg, Linn

AU - Hallström, Björn M.

AU - Lindskog, Cecilia

AU - Oksvold, Per

AU - Mardinoglu, Adil

AU - Sivertsson, Åsa

AU - Kampf, Caroline

AU - Sjöstedt, Evelina

AU - Asplund, Anna

AU - Olsson, IngMarie

AU - Edlund, Karolina

AU - Lundberg, Emma

AU - Navani, Sanjay

AU - Szigyarto, Cristina Al-Khalili

AU - Odeberg, Jacob

AU - Djureinovic, Dijana

AU - Takanen, Jenny Ottosson

AU - Hober, Sophia

AU - Alm, Tove

AU - Edqvist, Per-Henrik

AU - Berling, Holger

AU - Tegel, Hanna

AU - Mulder, Jan

AU - Rockberg, Johan

AU - Nilsson, Peter

AU - Schwenk, Jochen M.

AU - Hamsten, Marica

AU - von Feilitzen, Kalle

AU - Forsberg, Mattias

AU - Persson, Lukas

AU - Johansson, Fredric

AU - Zwahlen, Martin

AU - von Heijne, Gunnar

AU - Nielsen, Jens

AU - Pontén, Fredrik

PY - 2015

Y1 - 2015

N2 - Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.

AB - Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.

U2 - 10.1126/science.1260419

DO - 10.1126/science.1260419

M3 - Journal article

C2 - 25613900

VL - 347

SP - 394-

JO - Science

JF - Science

SN - 0036-8075

IS - 6220

M1 - 1260419

ER -

Tissue-based map of the human proteome

Abstract

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