Tissue-based biosensor for monitoring the antioxidant effect of orally administered drugs in the intestine

For a better understanding of the effect of drugs and their interaction with cells and tissues, there is a need for in vitro and ex vivo model systems which enables studying these events. There are several in vitro methods available to evaluate the antioxidant activity; however, these methods do not factor in the complex in vivo physiology. Here we present an intestinal tissue modified oxygen electrode, used for the detection of the antioxidant effect of orally administered drugs in the presence of H₂O₂. Antioxidants are essential in the defense against oxidative stress, more specifically against reactive oxygen species such as H₂O₂. Due to the presence of native catalase in the intestine, with the tissue-based biosensor we were able to detect H₂O₂ in the range between 50 and 500 µM. The reproducibility of the sensor based on the calculated relative standard deviations was 15 ± 6%. We found that the O₂ production by catalase from H₂O₂ was reduced in the presence of a well-known antioxidant, quinol. This indirectly detected antioxidant activity was also observed in the case of orally administered drugs with a reported anti-inflammatory effect such as mesalazine and paracetamol, while no antioxidant activity was recorded with aspirin and metformin.