The objective of the project was to investigate the time-dependent batch growth effects of oxidative environmental conditions on protein ki nase (PK) and phosphatase (PP) deletion mutants and relevant wild type strains of Saccharomyces cerevisiae . To achieve this goal, 44 different PK and PP mutants were selected for their known activities in various stress response pathways, including oxidative stress, and were investigated for their response to oxidative stress. Hydrogen peroxide was us ed as the oxidizing agent at a number of different concentrations ranging from mild to moderate stress (0.25, 0.50 and 1.0 mM). Understanding the growth physiology of S. cerevisiae allows us to estimate the link between genotype and stress-response phenotype. Growth physiology parameters, such as growth rate, diauxic shift times, stress-induced stasis times, were measured in fermentative batch cultures as important in dicators of cellular fitness. We used a high-throughput microfermentation system (BioLector, m2p-labs) to retrieve these indicators of cellular fitness and perform our batch fermentations.
|Publication status||Published - 2013|
|Event||26th International Conference on Yeast Genetics and Molecular Biology - Frankfurt Main, Germany|
Duration: 29 Aug 2013 → 3 Sep 2013
|Conference||26th International Conference on Yeast Genetics and Molecular Biology|
|Period||29/08/2013 → 03/09/2013|