Time dependent physiological characterization of yeast oxidative stress response and growth modulation of protein kinase/phosphatase mutants

Ali Altintas, Christopher Workman

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

    Abstract

    The objective of the project was to investigate the time-dependent batch growth effects of oxidative environmental conditions on protein ki nase (PK) and phosphatase (PP) deletion mutants and relevant wild type strains of Saccharomyces cerevisiae . To achieve this goal, 44 different PK and PP mutants were selected for their known activities in various stress response pathways, including oxidative stress, and were investigated for their response to oxidative stress. Hydrogen peroxide was us ed as the oxidizing agent at a number of different concentrations ranging from mild to moderate stress (0.25, 0.50 and 1.0 mM). Understanding the growth physiology of S. cerevisiae allows us to estimate the link between genotype and stress-response phenotype. Growth physiology parameters, such as growth rate, diauxic shift times, stress-induced stasis times, were measured in fermentative batch cultures as important in dicators of cellular fitness. We used a high-throughput microfermentation system (BioLector, m2p-labs) to retrieve these indicators of cellular fitness and perform our batch fermentations.
    Original languageEnglish
    JournalYeast
    Volume30
    Issue numberS1
    Pages (from-to)156-156
    ISSN0749-503X
    DOIs
    Publication statusPublished - 2013
    Event26th International Conference on Yeast Genetics and Molecular Biology - Frankfurt Main, Germany
    Duration: 29 Aug 20133 Sep 2013

    Conference

    Conference26th International Conference on Yeast Genetics and Molecular Biology
    CountryGermany
    CityFrankfurt Main
    Period29/08/201303/09/2013

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