Abstract
DNA vaccine against viral haemorrhagic septicaemia (VHS-DNA vaccine) induces effective and long-lasting protection in rainbow trout. The protective mechanisms induced by this vaccine start a few days post-vaccination when systemic innate responses are triggered. The specific adaptive immunity replaces the non-specific innate immunity a few weeks post-vaccination, which can protect the immunized fish during the whole farmed cycle.
The strong early responses induced by VHS-DNA vaccine could be the key to paving the way for an effective, specific, and long-lasting protection. Therefore, several studies have focused on dissecting the early response by analyzing the differentially expressed genes in different tissues. However, it is known that there is a low correlation between transcriptomic and proteomic (functional) analysis because of the post-transcriptional/translational regulations. Thus, it is not completely correct to explain biological processes by the results of gene expression analysis.
In this study, we performed a proteomic analysis using quantitative mass spectrometry of different tissues (muscle, liver, spleen, head kidney, blood cells, and thymus) of fish vaccinated with the VHS-DNA vaccine. Additionally, several sampling time points were done to have an overview of the kinetics of the mechanism involved. The differentially expressed proteins in each condition were used to perform pathways and network analysis to discover biological processes that could explain the high efficacy of this vaccine. These results could be used to compare and understand the mechanisms induced by other vaccines.
The strong early responses induced by VHS-DNA vaccine could be the key to paving the way for an effective, specific, and long-lasting protection. Therefore, several studies have focused on dissecting the early response by analyzing the differentially expressed genes in different tissues. However, it is known that there is a low correlation between transcriptomic and proteomic (functional) analysis because of the post-transcriptional/translational regulations. Thus, it is not completely correct to explain biological processes by the results of gene expression analysis.
In this study, we performed a proteomic analysis using quantitative mass spectrometry of different tissues (muscle, liver, spleen, head kidney, blood cells, and thymus) of fish vaccinated with the VHS-DNA vaccine. Additionally, several sampling time points were done to have an overview of the kinetics of the mechanism involved. The differentially expressed proteins in each condition were used to perform pathways and network analysis to discover biological processes that could explain the high efficacy of this vaccine. These results could be used to compare and understand the mechanisms induced by other vaccines.
| Original language | English |
|---|---|
| Title of host publication | 21st International Conference on Diseases of Fish and Shellfish: Abstract Book |
| Number of pages | 1 |
| Publisher | European Association of Fish Pathologists |
| Publication date | 2023 |
| Pages | 230-230 |
| Publication status | Published - 2023 |
| Event | 21st International Conference on Diseases of Fish and Shellfish - P&J Live Aberdeen, Aberdeen, United Kingdom Duration: 11 Sept 2023 → 14 Sept 2023 Conference number: 21 |
Conference
| Conference | 21st International Conference on Diseases of Fish and Shellfish |
|---|---|
| Number | 21 |
| Location | P&J Live Aberdeen |
| Country/Territory | United Kingdom |
| City | Aberdeen |
| Period | 11/09/2023 → 14/09/2023 |
