Three-dimensional positioning with optofluidic microscope

Asger Laurberg Vig, Rodolphe Marie, Eric Jensen, Anders Kristensen

    Research output: Contribution to journalConference articleResearch


    This paper reports on-chip based optical detection with three-dimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1 μm, 2.1 μm, 3 μm and 4 μm is measured using the OFM readout. These results are bench marked against those obtained with a PFF device using a conventional fluorescence microscope as readout. The size separated microspheres are detected by OFM with an accuracy of ≤ 0.92 μm. The position in the height of the channel and the velocity of the separated microspheres are detected with an accuracy of 1.4 μm and 0.08 mm/s respectively. Throughout the measurements of the height and velocity distribution, the microspheres are observed to move towards the center of the channel in regard to its height.
    Original languageEnglish
    JournalProceedings of SPIE - The International Society for Optical Engineering
    Pages (from-to)77621X
    Publication statusPublished - 2010
    EventSPIE Optics and Photonics Annual Meeting: Optical Trapping and Optical Micromanipulation VII - San Diego, CA, United States
    Duration: 1 Aug 20105 Aug 2010


    ConferenceSPIE Optics and Photonics Annual Meeting
    Country/TerritoryUnited States
    CitySan Diego, CA


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