This work studies the tension fatigue damage progression of a uni-directional glass fibre composite made from a non-crimp fabric similar to those used for the main load carrying parts of a wind turbine blade. The spatial damage progression in a chosen region of a test specimen is monitored on a micro-structural scale by ex-situ X-ray computed tomography. The centimetre sized specimen remains uncut during the ex-situ experiment. The experimental results indicate that uni-directional fibre fractures initiate from matrix cracks related to the structure of the fabric: first in the thin off-axis backing bundles at triple cross-over regions where the ±45∘ and 90∘ backing bundles intersect each other and lie close to a uni-directional bundle, and later followed by damage initiation in the other cross-over regions. Uni-directional fibre fractures were seen to increase in number with increasing number of cycles, and mainly progress in the thickness direction of uni-directional bundles (away from the backing bundles). Furthermore, the crack face separation of individual broken uni-directional fibres was observed to gradually increase with an increasing number of cycles. The progression path of the uni-directional fibre fractures was seen to be very dependent on the local backing bundle arrangement.