Thread-Like Radical-Polymerization via Autonomously Propelled (TRAP) Bots

Sarvesh Kumar Srivastava*, Fatemeh Ajalloueian, Anja Boisen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Micromotor-mediated synthesis of thread-like hydrogel microstructures in an aqueous environment is presented. The study utilizes a catalytic micromotor assembly (owing to the presence of a Pt layer), with an on-board chemical reservoir (i.e., polymerization mixture), toward thread-like radical-polymerization via autonomously propelled bots (i.e., TRAP bots). Synergistic coupling of catalytically active Pt layer, together with radical initiators (H2O2 and FeCl3 (III)), and PEGDA monomers preloaded into the TRAP bot, results in the polymerization of monomeric units into elongated thread-like hydrogel polymers coupled with self-propulsion. Interestingly, polymer generation via TRAP bots can also be triggered in the absence of hydrogen peroxide for cellular/biomedical application. The resulting polymeric hydrogel microstructures are able to entrap living cells (NIH 3T3 fibroblast cells), and are easily separable via a centrifugation or magnetic separation (owing to the presence of a Ni layer). The cellular biocompatibility of TRAP bots is established via a LIVE/DEAD assay and MTS cell proliferation assay (7 days observation). This is the first study demonstrating real-time in situ hydrogel polymerization via an artificial microswimmer, capable of enmeshing biotic/abiotic microobjects in its reaction environment, and lays a strong foundation for advanced applications in cell/tissue engineering, drug delivery, and cleaner technologies.

Original languageEnglish
Article number1901573
JournalAdvanced Materials
Volume31
Issue number30
ISSN0935-9648
DOIs
Publication statusPublished - 2019

Keywords

  • biocompatible micromotors
  • in situ polymerization
  • live cell trapping
  • PEGDA hydrogel
  • remote tissue culture

Fingerprint Dive into the research topics of 'Thread-Like Radical-Polymerization via Autonomously Propelled (TRAP) Bots'. Together they form a unique fingerprint.

Cite this