Thermophoretic forces on DNA measured with a single-molecule spring balance

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    Abstract

    We stretch a single DNA molecule with thermophoretic forces and measure these forces with a spring balance: the DNA molecule itself. It is an entropic spring which we calibrate, using as a benchmark its Brownian motion in the nanochannel that contains and prestretches it. This direct measurement of the thermophoretic force in a static configuration finds forces up to 130 fN. This is eleven times stronger than the force experienced by the same molecule in the same thermal gradient in bulk, where the molecule shields itself. Our stronger forces stretch the middle of the molecule up to 80% of its contour length. We find the Soret coefficient per unit length of DNA at various ionic strengths. It agrees, with novel precision, with results obtained in bulk for DNA too short to shield itself and with the thermodynamic model of thermophoresis.
    Original languageEnglish
    JournalPhysical Review Letters
    Volume113
    Issue number26
    Pages (from-to)268301
    Number of pages5
    ISSN0031-9007
    DOIs
    Publication statusPublished - 2014

    Bibliographical note

    © 2014 American Physical Society

    Cite this

    @article{28330289a7f04d08a9e4c1e68a5af80e,
    title = "Thermophoretic forces on DNA measured with a single-molecule spring balance",
    abstract = "We stretch a single DNA molecule with thermophoretic forces and measure these forces with a spring balance: the DNA molecule itself. It is an entropic spring which we calibrate, using as a benchmark its Brownian motion in the nanochannel that contains and prestretches it. This direct measurement of the thermophoretic force in a static configuration finds forces up to 130 fN. This is eleven times stronger than the force experienced by the same molecule in the same thermal gradient in bulk, where the molecule shields itself. Our stronger forces stretch the middle of the molecule up to 80{\%} of its contour length. We find the Soret coefficient per unit length of DNA at various ionic strengths. It agrees, with novel precision, with results obtained in bulk for DNA too short to shield itself and with the thermodynamic model of thermophoresis.",
    author = "Pedersen, {Jonas Nyvold} and L{\"u}scher, {Christopher James} and Rodolphe Marie and Thamdrup, {Lasse H{\o}jlund} and Anders Kristensen and Henrik Flyvbjerg",
    note = "{\circledC} 2014 American Physical Society",
    year = "2014",
    doi = "10.1103/PhysRevLett.113.268301",
    language = "English",
    volume = "113",
    pages = "268301",
    journal = "Physical Review Letters",
    issn = "0031-9007",
    publisher = "American Physical Society",
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    Thermophoretic forces on DNA measured with a single-molecule spring balance. / Pedersen, Jonas Nyvold; Lüscher, Christopher James; Marie, Rodolphe ; Thamdrup, Lasse Højlund; Kristensen, Anders; Flyvbjerg, Henrik.

    In: Physical Review Letters, Vol. 113, No. 26, 2014, p. 268301.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Thermophoretic forces on DNA measured with a single-molecule spring balance

    AU - Pedersen, Jonas Nyvold

    AU - Lüscher, Christopher James

    AU - Marie, Rodolphe

    AU - Thamdrup, Lasse Højlund

    AU - Kristensen, Anders

    AU - Flyvbjerg, Henrik

    N1 - © 2014 American Physical Society

    PY - 2014

    Y1 - 2014

    N2 - We stretch a single DNA molecule with thermophoretic forces and measure these forces with a spring balance: the DNA molecule itself. It is an entropic spring which we calibrate, using as a benchmark its Brownian motion in the nanochannel that contains and prestretches it. This direct measurement of the thermophoretic force in a static configuration finds forces up to 130 fN. This is eleven times stronger than the force experienced by the same molecule in the same thermal gradient in bulk, where the molecule shields itself. Our stronger forces stretch the middle of the molecule up to 80% of its contour length. We find the Soret coefficient per unit length of DNA at various ionic strengths. It agrees, with novel precision, with results obtained in bulk for DNA too short to shield itself and with the thermodynamic model of thermophoresis.

    AB - We stretch a single DNA molecule with thermophoretic forces and measure these forces with a spring balance: the DNA molecule itself. It is an entropic spring which we calibrate, using as a benchmark its Brownian motion in the nanochannel that contains and prestretches it. This direct measurement of the thermophoretic force in a static configuration finds forces up to 130 fN. This is eleven times stronger than the force experienced by the same molecule in the same thermal gradient in bulk, where the molecule shields itself. Our stronger forces stretch the middle of the molecule up to 80% of its contour length. We find the Soret coefficient per unit length of DNA at various ionic strengths. It agrees, with novel precision, with results obtained in bulk for DNA too short to shield itself and with the thermodynamic model of thermophoresis.

    U2 - 10.1103/PhysRevLett.113.268301

    DO - 10.1103/PhysRevLett.113.268301

    M3 - Journal article

    VL - 113

    SP - 268301

    JO - Physical Review Letters

    JF - Physical Review Letters

    SN - 0031-9007

    IS - 26

    ER -