Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation

Rasmus Krogh Norrild, Nicola Vettore, Alberto Coden, Wei-Feng Xue, Alexander K. Buell*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. We use quartz crystal microbalance (QCM) and develop novel temperature perturbation experiments based on differential scanning fluorimetry (DSF) to measure the temperature dependence of the fibril growth and dissociation rates, allowing us to quantitatively describe the thermodynamic stability of PI3K-SH3 amyloid fibrils between 10 and 75°C.
Original languageEnglish
Article number106549
JournalBiophysical Chemistry
Number of pages11
Publication statusPublished - 2021


  • Amyloid
  • Kinetics
  • Thermodynamics
  • Non-equilibrium system
  • Biosensing
  • Differential scanning fluorimetry
  • Quartz crystal microbalance


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