Thermochemical pretreatments for enhancing succinic acid production from industrial hemp (Cannabis sativa L.)

Ingólfur Bragi Gunnarsson, Mariusz Kuglarz, Dimitar Borisov Karakashev, Irini Angelidaki

Research output: Contribution to journalJournal articleResearchpeer-review


The aim of this study was to develop an efficient thermochemical method for treatment of industrial hemp biomass, in order to increase its bioconversion to succinic acid. Industrial hemp was subjected to various thermochemical pretreatments using 0-3% H2SO4, NaOH or H2O2 at 121-180°C prior to enzymatic hydrolysis. The influence of the different pretreatments on hydrolysis and succinic acid production by Actinobacillus succinogenes 130Z was investigated in batch mode, using anaerobic bottles and bioreactors. Enzymatic hydrolysis and fermentation of hemp material pretreated with 3% H2O2 resulted in the highest overall sugar yield (73.5%), maximum succinic acid titer (21.9gL-1), as well as the highest succinic acid yield (83%). Results obtained clearly demonstrated the impact of different pretreatments on the bioconversion efficiency of industrial hemp into succinic acid.
Original languageEnglish
JournalBioresource Technology
Pages (from-to)58-66
Number of pages9
Publication statusPublished - 2015


  • Hydrogen peroxide
  • Industrial hemp
  • Pretreatment
  • Succinic acid
  • Bioconversion
  • Hemp
  • Hydrolysis
  • Actinobacillus succinogenes
  • Batch modes
  • Cannabis sativa
  • Pre-Treatment
  • Pre-treatments
  • Succinic acids
  • Thermochemical method
  • Enzymatic hydrolysis


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