Thermal injury induces impaired function in polymorphonuclear neutrophil granulocytes and reduced control of burn wound infection

H. Calum, C. Moser, P. O. Jensen, L. Christophersen, D. S. Maling, Maria van Gennip, Thomas Bjarnsholt, H. P. Hougen, Michael Christian Givskov, G. K. Jacobsen, N. Hoiby

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Severe thermal injury induces immunosuppression, involving all parts of the immune system, especially when large fractions of the total body surface area are affected. An animal model was established to characterize the burn-induced immunosuppression. In our novel mouse model a 6% third-degree burn injury was induced in mice with a hot-air blower. The third-degree burn was confirmed histologically. The mice were allocated into five groups: control, shave, burn, infection and burn infection group. At 48 h, a decline in the concentration of peripheral blood leucocytes was observed in the group of mice with burn wound. The reduction was ascribed to the decline in concentration of polymorphonuclear neutrophil leucocytes and monocytes. When infecting the skin with Pseudomonas aeruginosa, a dissemination of bacteria was observed only in the burn wound group. Histological characterization of the skin showed a more polymorphonuclear neutrophil granulocytes (PMNs)-dominated inflammation in the group of mice with infected burn wound compared with the with burn wound group. In contrast, a higher degree of inflammation was observed in the burn wound group compared with the group of mice with infected burn wound. Furthermore, the oxidative burst and the phagocytic capacity of the PMNs were reduced in the group of mice with burn wound. Using this novel mouse model of thermal injury a decline of peripheral leucocytes was observed, whereas the increased local inflammatory response at the site of infection showed reduced capacity to contain and eliminate the infection.
    Original languageEnglish
    JournalClinical and Experimental Immunology
    Volume156
    Issue number1
    Pages (from-to)102-110
    ISSN0009-9104
    DOIs
    Publication statusPublished - 2009

    Keywords

    • PMNs
    • thermal injury
    • animal model
    • Pseudomonas aeruginosa
    • phagocytosis
    • oxidative burst

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