Theoretical and experimental comparison of microelectrode sensing configurations for impedimetric cell monitoring

M. Carminati, Claudia Caviglia, Arto Heiskanen, Marco Vergani, G. Ferrari, M. Sampietro, Thomas Lars Andresen, Jenny Emnéus

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. These widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10 μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. The characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100 kHz vs. 24 kHz) shifting over time. © 2014 Taylor & Francis Group.
    Original languageEnglish
    Title of host publicationLecture Notes on Impedance Spectroscopy : Measurement, Modeling and Applications
    Volume4
    Publication date2013
    Pages75-82
    Chapter9
    ISBN (Print)978-1-138-00140-4
    ISBN (Electronic)978-1-315-79567-6
    DOIs
    Publication statusPublished - 2013
    Event6th International Workshop on Impedance Spectroscopy - Chemnitz, Germany
    Duration: 25 Sep 201327 Sep 2013
    Conference number: 6

    Conference

    Conference6th International Workshop on Impedance Spectroscopy
    Number6
    CountryGermany
    CityChemnitz
    Period25/09/201327/09/2013

    Keywords

    • Cell adhesion
    • Cell culture
    • Cell proliferation
    • Spectroscopy
    • Voltage regulators
    • Microelectrodes

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