Abstract
A theoretical and experimental comparison between vertical and coplanar interdigitated sensing configurations for impedimetric cell growth tracking is presented. These widely-adopted approaches are quantitatively compared on the same cell populations and on the same 10 μm interdigitated microelectrodes using a versatile custom-made monitoring platform including a 24-channel miniaturized potentiostat. The characterization of bare microelectrodes in buffer and tracking experiments with HeLa cells over 16 hours demonstrate that the coplanar configuration provides a higher sensitivity to cell adhesion and spreading (Cell Index = 1.6 vs. 0.4) albeit at a higher frequency of maximum sensitivity (100 kHz vs. 24 kHz) shifting over time. © 2014 Taylor & Francis Group.
| Original language | English |
|---|---|
| Title of host publication | Lecture Notes on Impedance Spectroscopy : Measurement, Modeling and Applications |
| Volume | 4 |
| Publication date | 2013 |
| Pages | 75-82 |
| Chapter | 9 |
| ISBN (Print) | 978-1-138-00140-4 |
| ISBN (Electronic) | 978-1-315-79567-6 |
| DOIs | |
| Publication status | Published - 2013 |
| Event | 6th International Workshop on Impedance Spectroscopy - Chemnitz, Germany Duration: 25 Sept 2013 → 27 Sept 2013 Conference number: 6 |
Conference
| Conference | 6th International Workshop on Impedance Spectroscopy |
|---|---|
| Number | 6 |
| Country/Territory | Germany |
| City | Chemnitz |
| Period | 25/09/2013 → 27/09/2013 |
Keywords
- Cell adhesion
- Cell culture
- Cell proliferation
- Spectroscopy
- Voltage regulators
- Microelectrodes