The YmdB phosphodiesterase is a global regulator of late adaptive responses in bacillus subtilis

Christine Diethmaier, Joseph A. Newman, Ákos T. Kovács, Volkhard Kaever, Christina Herzberg, Cecilia Rodrigues, Mirjam Boonstra, Oscar P. Kuipers, Richard J. Lewis*, Jörg Stülke

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the σD-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.

Original languageEnglish
JournalJournal of Bacteriology
Volume196
Issue number2
Pages (from-to)265-275
ISSN0021-9193
DOIs
Publication statusPublished - 2014
Externally publishedYes

Cite this

Diethmaier, C., Newman, J. A., Kovács, Á. T., Kaever, V., Herzberg, C., Rodrigues, C., Boonstra, M., Kuipers, O. P., Lewis, R. J., & Stülke, J. (2014). The YmdB phosphodiesterase is a global regulator of late adaptive responses in bacillus subtilis. Journal of Bacteriology, 196(2), 265-275. https://doi.org/10.1128/JB.00826-13