The value of ultrahigh resolution OCT in dermatology - delineating the dermo-epidermal junction, capillaries in the dermal papillae and vellus hairs

Niels Møller Israelsen*, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Podoleanu, Ole Bang

*Corresponding author for this work

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Abstract

Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epidermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epidermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis.In this work, we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology.In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR- OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR- OCT image has previously been reported, despite many publications within this field.For selected healthy volunteers, we investigate the dermal papillae and the vellus hairs and identify distinct features that only UHR- OCT can resolve. Differences are seen in tracing hairs of diameter below 20 mu m, and in imaging the dermal papillae where, when utilising the UHR- OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Original languageEnglish
JournalBiomedical Optics Express
Volume9
Issue number5
Pages (from-to)2225-2250
ISSN2156-7085
DOIs
Publication statusPublished - 2018

Cite this

@article{ed7082574bc74f68843f10c6cfc04898,
title = "The value of ultrahigh resolution OCT in dermatology - delineating the dermo-epidermal junction, capillaries in the dermal papillae and vellus hairs",
abstract = "Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epidermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epidermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis.In this work, we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology.In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR- OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR- OCT image has previously been reported, despite many publications within this field.For selected healthy volunteers, we investigate the dermal papillae and the vellus hairs and identify distinct features that only UHR- OCT can resolve. Differences are seen in tracing hairs of diameter below 20 mu m, and in imaging the dermal papillae where, when utilising the UHR- OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.",
author = "Israelsen, {Niels M{\o}ller} and Michael Maria and Mette Mogensen and Sophie Bojesen and Mikkel Jensen and Merete Haedersdal and Adrian Podoleanu and Ole Bang",
year = "2018",
doi = "10.1364/BOE.9.002240",
language = "English",
volume = "9",
pages = "2225--2250",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "Optical Society of America",
number = "5",

}

The value of ultrahigh resolution OCT in dermatology - delineating the dermo-epidermal junction, capillaries in the dermal papillae and vellus hairs. / Israelsen, Niels Møller; Maria, Michael; Mogensen, Mette; Bojesen, Sophie; Jensen, Mikkel; Haedersdal, Merete; Podoleanu, Adrian; Bang, Ole.

In: Biomedical Optics Express, Vol. 9, No. 5, 2018, p. 2225-2250.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The value of ultrahigh resolution OCT in dermatology - delineating the dermo-epidermal junction, capillaries in the dermal papillae and vellus hairs

AU - Israelsen, Niels Møller

AU - Maria, Michael

AU - Mogensen, Mette

AU - Bojesen, Sophie

AU - Jensen, Mikkel

AU - Haedersdal, Merete

AU - Podoleanu, Adrian

AU - Bang, Ole

PY - 2018

Y1 - 2018

N2 - Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epidermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epidermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis.In this work, we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology.In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR- OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR- OCT image has previously been reported, despite many publications within this field.For selected healthy volunteers, we investigate the dermal papillae and the vellus hairs and identify distinct features that only UHR- OCT can resolve. Differences are seen in tracing hairs of diameter below 20 mu m, and in imaging the dermal papillae where, when utilising the UHR- OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

AB - Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epidermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epidermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis.In this work, we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology.In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR- OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR- OCT image has previously been reported, despite many publications within this field.For selected healthy volunteers, we investigate the dermal papillae and the vellus hairs and identify distinct features that only UHR- OCT can resolve. Differences are seen in tracing hairs of diameter below 20 mu m, and in imaging the dermal papillae where, when utilising the UHR- OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

U2 - 10.1364/BOE.9.002240

DO - 10.1364/BOE.9.002240

M3 - Journal article

C2 - 29760984

VL - 9

SP - 2225

EP - 2250

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 5

ER -