The development of cell factories for the production of chemicals has traditionally relied on measurements of product metabolite titers to assess the performance of genetically manipulated strains. With the development of improved metabolomics and targeted metabolite profiling methods, these broader measurements of the cellular metabolic state are now becoming part of the toolbox used to characterize cell factories. In this review we briefly summarize the benefits and challenges of global metabolomics and targeted metabolite profiling methods and discuss the application of these methods in both pathway discovery and cell factory engineering. We focus particularly on exploring the potential of global metabolomics to complement more traditional targeted methods. We conclude the review by discussing emerging trends in metabolomics and how these developments can aid the engineering of better cell factories in the future.
Harrison, S. J., & Herrgard, M. (2013). The Uses and Future Prospects of Metabolomics and Targeted Metabolite Profiling in Cell Factory Development. Industrial Biotechnology, 9(4), 196-202. https://doi.org/10.1089/ind.2013.0008