The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers

Hugo Ahlm Grønlund; Charlotta Löfström; Jens Bue Helleskov; Jeffrey Hoorfar

doi:10.1007/s12161-009-9103-2

The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers

Hugo Ahlm Grønlund, Charlotta Löfström, Jens Bue Helleskov, Jeffrey Hoorfar

National Food Institute

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Abstract

Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool.
After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.

Original language	English
Journal	Food Analytical Methods
Volume	3
Issue number	2
Pages (from-to)	116–119
ISSN	1936-9751
DOIs	https://doi.org/10.1007/s12161-009-9103-2
Publication status	Published - Jun 2010

Keywords

PCR
Thermocycler
Thermal Cycler
Infrared Thermography
Validation

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10.1007/s12161-009-9103-2

Gronlund et al 2010 pre print versionSubmitted manuscript, 367 KB

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title = "The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers",

abstract = "Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool.After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.",

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AU - Hoorfar, Jeffrey

PY - 2010/6

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AB - Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool.After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.

KW - PCR

KW - Thermocycler

KW - Thermal Cycler

KW - Infrared Thermography

KW - Validation

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The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers

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