The use of archived tags in retrospective genetic analysis of fish

Sara Bonanomi, Nina Overgaard Therkildsen, Rasmus Berg Hedeholm, Jakob Hemmer Hansen, Einar Eg Nielsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish
Original languageEnglish
JournalMolecular Ecology Resources
Volume14
Issue number3
Pages (from-to)616-621
ISSN1755-098X
DOIs
Publication statusPublished - 2014

Cite this

Bonanomi, Sara ; Therkildsen, Nina Overgaard ; Hedeholm, Rasmus Berg ; Hansen, Jakob Hemmer ; Eg Nielsen, Einar. / The use of archived tags in retrospective genetic analysis of fish. In: Molecular Ecology Resources. 2014 ; Vol. 14, No. 3. pp. 616-621.
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The use of archived tags in retrospective genetic analysis of fish. / Bonanomi, Sara; Therkildsen, Nina Overgaard; Hedeholm, Rasmus Berg; Hansen, Jakob Hemmer; Eg Nielsen, Einar.

In: Molecular Ecology Resources, Vol. 14, No. 3, 2014, p. 616-621.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Therkildsen, Nina Overgaard

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AU - Hansen, Jakob Hemmer

AU - Eg Nielsen, Einar

PY - 2014

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AB - Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish

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