The temporal, PFGE and resistance pattern associations suggest that poultry products are only a minor source of human infections in Western Finland

Ulrike Lyhs, Marianne Katzav, Pauliina Isohanni, Helmi Heiska, Riitta Maijala

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Abstract

In order to compare human and retail poultry meat thermophilic Campylobacter isolates originating in a regional area in Western Finland, minimum inhibitory concentration (MICs) for six antimicrobials (96 isolates) and pulsed-field gel electrophoresis (PFGE) (102 isolates) were analysed Campylobacter spp were detected in 10 5% Out of 305 fresh poultry products studied, 29 (90.5%) isolates were identified as Campylobacter jejum. Among the 70 human isolates, 66 (94.3%) isolates were identified as C jejuni. Only one C jejuni domestic poultry isolate showed resistance (ampicillin), whereas domestic human C jejuni isolates were more commonly resistant to ciprofloxacin, nalidixic acid, ampicillin and tetracycline The resistance in foreign human isolates was significantly more common than among domestic isolates. PFGE analysis with KpnI restriction enzyme resulted in 59 different PFGE types among the poultry and human isolates Three types were detected first in poultry meat and thereafter during the following month in domestic human samples, whereas the other conjoint types were detected only after many months This study Suggests that poultry products play only a minor role in human campylobacteriosis in the Study area and that the resistance found in domestic human isolates is not likely related to retail Poultry meat products. (C) 2009 Elsevier Ltd All rights reserved.
Original languageEnglish
JournalFood Microbiology
Volume27
Issue number2
Pages (from-to)311-315
ISSN0740-0020
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Food Science
  • Microbiology
  • Antimicrobial resistance
  • Campylobacter
  • Incidence
  • PFGE
  • Poultry meat
  • Source attribution
  • animal
  • antibiotic resistance
  • article
  • Campylobacter jejuni
  • campylobacteriosis
  • feces
  • Finland
  • food poisoning
  • genetics
  • human
  • isolation and purification
  • meat
  • microbiology
  • poultry
  • pulsed field gel electrophoresis
  • Animals
  • Campylobacter Infections
  • Drug Resistance, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Feces
  • Foodborne Diseases
  • Humans
  • Meat Products
  • Poultry
  • BIOTECHNOLOGY
  • FOOD
  • MICROBIOLOGY
  • FIELD GEL-ELECTROPHORESIS
  • CAMPYLOBACTER-JEJUNI
  • COLI
  • CHICKEN
  • CONTAMINATION
  • SUSCEPTIBILITIES
  • SALMONELLA
  • QUINOLONE
  • STRAINS
  • WHOLE
  • Finland Europe Palearctic region
  • Aves Vertebrata Chordata Animalia (Animals, Birds, Chordates, Nonhuman Vertebrates, Vertebrates) - Galliformes [85536] chicken common turkey common
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Aerobic Helical or Vibrioid Gram-Negatives [06210] Campylobacter jejuni species pathogen, contaminant
  • Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common host
  • ampicillin 69-53-4 antibacterial-drug, antiinfective-drug
  • ciprofloxacin 85721-33-1 antibacterial-drug, antiinfective-drug
  • KpnI restriction enzyme
  • nalidixic acid 389-08-2 antibacterial-drug, antiinfective-drug
  • tetracycline 60-54-8 enzyme inhibitor-drug, antiinfective-drug, antibacterial-drug
  • 10060, Biochemistry studies - General
  • 12502, Pathology - General
  • 12512, Pathology - Therapy
  • 13502, Food technology - General and methods
  • 13516, Food technology - Meats and meat by-products
  • 31000, Physiology and biochemistry of bacteria
  • 36002, Medical and clinical microbiology - Bacteriology
  • 38502, Chemotherapy - General, methods and metabolism
  • 38504, Chemotherapy - Antibacterial agents
  • Foods
  • Human Medicine
  • Infection
  • Methods and Techniques
  • campylobacteriosis Campylobacter Infections (MeSH) bacterial disease
  • drug resistance
  • poultry product meat product
  • stool sample
  • Medical Sciences
  • pulse field gel electrophoresis PFGE electrophoretic techniques, laboratory techniques
  • CAMPYLOBACTER
  • DISEASES
  • EPIDEMIOLOGY
  • FINLAND
  • FOOD SAFETY ANIMAL FOODS
  • POULTRY MEAT
  • POULTRY PRODUCTS
  • Meat, poultry and game
  • Poultry and products

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