The susceptibility of silver crucian carp (Carassius auratus langsdorfii) to infection with koi herpesvirus (KHV)

Hyoung Jun Kim, Se Ryun Kwon, Niels Jørgen Olesen, Kei Yuasa*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Koi herpesvirus (KHV) infections cause high mortality in carp (Cyprinus carpio). This study compared the susceptibility of silver crucian carp (Carassius auratus langsdorfii), also called ginbuna, and koi carp to KHV infection. Silver crucian carp and koi carp were challenged with KHV by both intraperitoneal injection and immersion, respectively, and kept in tanks at 22°C. All KHV-exposed koi carp died within 14 days post-infection (dpi), whereas no clinics nor mortality was observed in the KHV-exposed silver crucian carp. KHV DNA was detected in both koi and silver crucian carp shortly after infection. At 7 dpi, the copy numbers of KHV genome were increased in koi carp but decreased in silver crucian carp. Using reverse transcriptase PCR, KHV mRNA was detected in koi carp but not in silver crucian carp. Cell cultivation on common carp brain (CCB) cell samples from koi carp caused KHV-associated cytopathic effects in CCB cells. Therefore, we concluded that KHV replicated in koi carp but not in silver crucian carp and that silver crucian carp is not susceptible to infection with KHV.
Original languageEnglish
JournalJournal of Fish Diseases
Volume42
Issue number10
Pages (from-to)1333-1340
ISSN0140-7775
DOIs
Publication statusPublished - 2019

Keywords

  • Ginbuna
  • Koi herpesvirus (KHV)
  • mRNA-specific RT-PCR with KHV
  • Real-time PCR
  • Silver crucian carp
  • Susceptibility

Cite this

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title = "The susceptibility of silver crucian carp (Carassius auratus langsdorfii) to infection with koi herpesvirus (KHV)",
abstract = "Koi herpesvirus (KHV) infections cause high mortality in carp (Cyprinus carpio). This study compared the susceptibility of silver crucian carp (Carassius auratus langsdorfii), also called ginbuna, and koi carp to KHV infection. Silver crucian carp and koi carp were challenged with KHV by both intraperitoneal injection and immersion, respectively, and kept in tanks at 22°C. All KHV-exposed koi carp died within 14 days post-infection (dpi), whereas no clinics nor mortality was observed in the KHV-exposed silver crucian carp. KHV DNA was detected in both koi and silver crucian carp shortly after infection. At 7 dpi, the copy numbers of KHV genome were increased in koi carp but decreased in silver crucian carp. Using reverse transcriptase PCR, KHV mRNA was detected in koi carp but not in silver crucian carp. Cell cultivation on common carp brain (CCB) cell samples from koi carp caused KHV-associated cytopathic effects in CCB cells. Therefore, we concluded that KHV replicated in koi carp but not in silver crucian carp and that silver crucian carp is not susceptible to infection with KHV.",
keywords = "Ginbuna, Koi herpesvirus (KHV), mRNA-specific RT-PCR with KHV, Real-time PCR, Silver crucian carp, Susceptibility",
author = "Kim, {Hyoung Jun} and Kwon, {Se Ryun} and Olesen, {Niels J{\o}rgen} and Kei Yuasa",
year = "2019",
doi = "10.1111/jfd.13054",
language = "English",
volume = "42",
pages = "1333--1340",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
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}

The susceptibility of silver crucian carp (Carassius auratus langsdorfii) to infection with koi herpesvirus (KHV). / Kim, Hyoung Jun; Kwon, Se Ryun; Olesen, Niels Jørgen; Yuasa, Kei.

In: Journal of Fish Diseases, Vol. 42, No. 10, 2019, p. 1333-1340.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - The susceptibility of silver crucian carp (Carassius auratus langsdorfii) to infection with koi herpesvirus (KHV)

AU - Kim, Hyoung Jun

AU - Kwon, Se Ryun

AU - Olesen, Niels Jørgen

AU - Yuasa, Kei

PY - 2019

Y1 - 2019

N2 - Koi herpesvirus (KHV) infections cause high mortality in carp (Cyprinus carpio). This study compared the susceptibility of silver crucian carp (Carassius auratus langsdorfii), also called ginbuna, and koi carp to KHV infection. Silver crucian carp and koi carp were challenged with KHV by both intraperitoneal injection and immersion, respectively, and kept in tanks at 22°C. All KHV-exposed koi carp died within 14 days post-infection (dpi), whereas no clinics nor mortality was observed in the KHV-exposed silver crucian carp. KHV DNA was detected in both koi and silver crucian carp shortly after infection. At 7 dpi, the copy numbers of KHV genome were increased in koi carp but decreased in silver crucian carp. Using reverse transcriptase PCR, KHV mRNA was detected in koi carp but not in silver crucian carp. Cell cultivation on common carp brain (CCB) cell samples from koi carp caused KHV-associated cytopathic effects in CCB cells. Therefore, we concluded that KHV replicated in koi carp but not in silver crucian carp and that silver crucian carp is not susceptible to infection with KHV.

AB - Koi herpesvirus (KHV) infections cause high mortality in carp (Cyprinus carpio). This study compared the susceptibility of silver crucian carp (Carassius auratus langsdorfii), also called ginbuna, and koi carp to KHV infection. Silver crucian carp and koi carp were challenged with KHV by both intraperitoneal injection and immersion, respectively, and kept in tanks at 22°C. All KHV-exposed koi carp died within 14 days post-infection (dpi), whereas no clinics nor mortality was observed in the KHV-exposed silver crucian carp. KHV DNA was detected in both koi and silver crucian carp shortly after infection. At 7 dpi, the copy numbers of KHV genome were increased in koi carp but decreased in silver crucian carp. Using reverse transcriptase PCR, KHV mRNA was detected in koi carp but not in silver crucian carp. Cell cultivation on common carp brain (CCB) cell samples from koi carp caused KHV-associated cytopathic effects in CCB cells. Therefore, we concluded that KHV replicated in koi carp but not in silver crucian carp and that silver crucian carp is not susceptible to infection with KHV.

KW - Ginbuna

KW - Koi herpesvirus (KHV)

KW - mRNA-specific RT-PCR with KHV

KW - Real-time PCR

KW - Silver crucian carp

KW - Susceptibility

U2 - 10.1111/jfd.13054

DO - 10.1111/jfd.13054

M3 - Journal article

VL - 42

SP - 1333

EP - 1340

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 10

ER -