The structure of the polysaccharide part of the LPS from Serratia marcescens serotype O19, including linkage region to the core and the residue at the non-reducing end

E. Vinogradov, Bent O. Petersen, Jens Øllgaard Duus, J. Radziejewska-Lebrecht

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The structure of the LPS from Serratia marcescens serotype O19 was investigated. Deamination of the LPS released the O-chain polysaccharide together with a fragment of the core oligosaccharide. The following structure of the product was determined by NMR spectroscopy, mass spectrometry, and chemical methods: [GRAPHICS] The main polymer consists of a repeating disaccharide V-U and is present on average of 18 units per chain as estimated by integration of signals in the NMR spectra. The residue O corresponds to the primer, which initiates biosynthesis of the O-chain, and an oligomer of a disaccharide R-S is an insert between the primer and the main polymer. The polysaccharide has a beta-Kdo residue at the non-reducing end a feature similar to that observed previously in the LPS from Klebsiella O12. (C) 2003 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalCarbohydrate Research
Volume338
Issue number23
Pages (from-to)2757-2761
Number of pages5
ISSN0008-6215
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • Carbohydrate Sequence
  • Lipopolysaccharides
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Sequence Data
  • Polymers
  • Polysaccharides
  • Polysaccharides, Bacterial
  • Serratia marcescens
  • Spectrometry, Mass, Electrospray Ionization
  • Biosynthesis
  • Mass spectrometry
  • Molecular structure
  • Nuclear magnetic resonance spectroscopy
  • Oligomers
  • bacterium lipopolysaccharide
  • disaccharide
  • oligomer
  • oligosaccharide
  • polysaccharide
  • article
  • biosynthesis
  • carbohydrate analysis
  • chemical analysis
  • chemical composition
  • controlled study
  • mass spectrometry
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • priority journal
  • serotype
  • structure analysis
  • Klebsiella
  • Serratia
  • Signal integration
  • Core
  • LPS
  • O-chain
  • X
  • ESIMS, electrospray ionization mass spectrometry
  • LPS, lipopolysaccharide
  • P, phosphate
  • Hep, l-glycero-d-manno-heptose
  • DD-Hep, d-glycero-d-manno-heptose
  • GalA, d-galacturonic acid
  • Kdo, 3-deoxy-d-manno-oct-2-ulosonic acid
  • Ko, d-glycero-d-talo-oct-2-ulosonic acid
  • 2,5-anhMan, 2,5-anhydro-d-mannose
  • Ara4N, 4-amino-4-deoxy-l-arabinose
  • BIOCHEMISTRY
  • CHEMISTRY,
  • KLEBSIELLA-PNEUMONIAE
  • LIPOPOLYSACCHARIDES
  • ANTIGENS
  • core
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Klebsiella genus Serratia marcescens species serotype O19
  • lipopolysaccharide linkage region, non-reducing end, polysaccharide, structure
  • 10060, Biochemistry studies - General
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • chemical method laboratory techniques
  • mass spectrometry laboratory techniques, spectrum analysis techniques
  • NMR spectroscopy laboratory techniques, spectrum analysis techniques
  • Biochemistry and Molecular Biophysics

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