The structure of the linkage between the O-specific polysaccharide and the core region of the lipopolysaccharide from Salmonella enterica serovar Typhimurium revisited

Maurien M. A. Olsthoorn, Bent O. Petersen, Jens Øllgaard Duus, Johan Haverkamp, Jane E. Thomas-Oates, Klaus Bock, Otto Holst

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Salmonella enterica sv. Typhimurium strain 1135 possesses smooth(S)-form lipopolysaccharide (LPS). Although the structures of the core region and the O-specific polysaccharide were investigated intensively between the 1960s and the 1980s, the structure of the linkage region between the O-chain and the core was not elucidated unequivocally. By using modern MS and high-field NMR spectroscopy for analysis of the isolated carbohydrate backbone of the LPS, it has been shown that it is a beta-d-Galp residue that links the first repeating unit of the O-specific polysaccharide to O-4 of the last d-Glcp residue of the core region. Interestingly, this particular d-Galp residue is alpha-linked in all following repeating units. The data are discussed with regard to the ligation of O-specific polysaccharide and core region during LPS biosynthesis.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume267
Issue number7
Pages (from-to)2014-2027
ISSN0014-2956
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Models, Molecular
  • Molecular Sequence Data
  • O Antigens
  • Salmonella enterica
  • bacterium lipopolysaccharide
  • carbohydrate
  • polysaccharide
  • article
  • bacterial outer membrane
  • biosynthesis
  • carbohydrate analysis
  • fast atom bombardment mass spectrometry
  • molecular model
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • priority journal
  • Salmonella typhimurium
  • strain difference
  • Bacteria (microorganisms)
  • Salmonella enterica subsp. enterica serovar Typhimurium
  • Typhimurium
  • Lipopolysaccharide
  • Mass spectrometry
  • NMR spectroscopy
  • O-specific polysaccharide
  • Salmonella enterica sv. Typhimurium
  • lipopolysaccharide
  • mass spectrometry
  • O‐specific polysaccharide
  • BIOCHEMISTRY
  • NMR-SPECTROSCOPY
  • OLIGOSACCHARIDE REGIONS
  • SEROGROUP-A
  • ANTIGEN
  • CONFORMATION
  • HYDROLYSIS
  • PROTEINS
  • SPECTRA
  • CHAIN
  • ACID
  • Salmonella enterica sv.
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Salmonella enterica serovar-Typhimurium, strain-1135
  • lipopolysaccharide biosynthesis, core region, smooth-form
  • 06502, Radiation biology - General
  • 10060, Biochemistry studies - General
  • 10502, Biophysics - General
  • 30000, Bacteriology, general and systematic
  • 31500, Genetics of bacteria and viruses
  • MS mass spectrometry analytical method
  • NMR spectroscopy analytical method
  • Biochemistry and Molecular Biophysics
  • Methods and Techniques
  • POLYSACCHARIDES

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