Abstract
Salmonella enterica sv. Typhimurium strain 1135 possesses smooth(S)-form lipopolysaccharide (LPS). Although the structures of the core region and the O-specific polysaccharide were investigated intensively between the 1960s and the 1980s, the structure of the linkage region between the O-chain and the core was not elucidated unequivocally. By using modern MS and high-field NMR spectroscopy for analysis of the isolated carbohydrate backbone of the LPS, it has been shown that it is a beta-d-Galp residue that links the first repeating unit of the O-specific polysaccharide to O-4 of the last d-Glcp residue of the core region. Interestingly, this particular d-Galp residue is alpha-linked in all following repeating units. The data are discussed with regard to the ligation of O-specific polysaccharide and core region during LPS biosynthesis.
Original language | English |
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Journal | European Journal of Biochemistry |
Volume | 267 |
Issue number | 7 |
Pages (from-to) | 2014-2027 |
ISSN | 0014-2956 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
Keywords
- Carbohydrate Conformation
- Carbohydrate Sequence
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Models, Molecular
- Molecular Sequence Data
- O Antigens
- Salmonella enterica
- bacterium lipopolysaccharide
- carbohydrate
- polysaccharide
- article
- bacterial outer membrane
- biosynthesis
- carbohydrate analysis
- fast atom bombardment mass spectrometry
- molecular model
- nonhuman
- nuclear magnetic resonance spectroscopy
- priority journal
- Salmonella typhimurium
- strain difference
- Bacteria (microorganisms)
- Salmonella enterica subsp. enterica serovar Typhimurium
- Typhimurium
- Lipopolysaccharide
- Mass spectrometry
- NMR spectroscopy
- O-specific polysaccharide
- Salmonella enterica sv. Typhimurium
- lipopolysaccharide
- mass spectrometry
- O‐specific polysaccharide
- BIOCHEMISTRY
- NMR-SPECTROSCOPY
- OLIGOSACCHARIDE REGIONS
- SEROGROUP-A
- ANTIGEN
- CONFORMATION
- HYDROLYSIS
- PROTEINS
- SPECTRA
- CHAIN
- ACID
- Salmonella enterica sv.
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Salmonella enterica serovar-Typhimurium, strain-1135
- lipopolysaccharide biosynthesis, core region, smooth-form
- 06502, Radiation biology - General
- 10060, Biochemistry studies - General
- 10502, Biophysics - General
- 30000, Bacteriology, general and systematic
- 31500, Genetics of bacteria and viruses
- MS mass spectrometry analytical method
- NMR spectroscopy analytical method
- Biochemistry and Molecular Biophysics
- Methods and Techniques
- POLYSACCHARIDES