The structure of the carbohydrate backbone of the lipopolysaccharide from Acinetobacter baumannii strain ATCC 19606

Evgeny V. Vinogradov, Jens Øllgaard Duus, Helmuth Brade, Otto Holst

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The chemical structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide (LPS) from Acinetobacter baumannii strain ATCC 19606 was investigated by chemical analysis and NMR spectroscopy of oligosaccharides obtained after deacylation or mild acid hydrolysis. From the combined information the following carbohydrate backbones can be deduced: [GRAPHICS] where R-1 = H and R-2 = alpha-Glcp-(1 --> 2)-beta-Glcp-(1 --> 4)-beta-Glcp-(1 --> 4)-beta-Glcp-(1 as major and R-1 = Ac and R-2 = H as minor products. All monosaccharides are D-configured. Also, smaller oligosaccharide phosphates were identified that are thought to represent degradation products of the above structures.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume269
Issue number2
Pages (from-to)422-430
ISSN0014-2956
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Acinetobacter
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chromatography, Gel
  • Mass Spectrometry
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • O Antigens
  • bacterium lipopolysaccharide
  • Acinetobacter baumannii
  • article
  • carbohydrate analysis
  • controlled study
  • deacetylation
  • degradation
  • hydrolysis
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • phosphorylation
  • priority journal
  • Bacteria (microorganisms)
  • Negibacteria
  • Core region
  • Lipopolysaccharide
  • NMR spectroscopy
  • Structural analysis
  • lipopolysaccharide
  • core region
  • structural analysis
  • BIOCHEMISTRY
  • O-ANTIGENIC POLYSACCHARIDE
  • GENUS-SPECIFIC EPITOPE
  • SEROLOGICAL CHARACTERIZATION
  • LIPID-A
  • HAEMOLYTICUS
  • CHLAMYDIA
  • ACID
  • OLIGOSACCHARIDE
  • TRACHOMATIS
  • NCTC-10305
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Neisseriaceae [06507] Acinetobacter baumannii strain-ATCC 19606
  • lipopolysaccharide carbohydrate backbone, structure
  • oligosaccharides
  • 10060, Biochemistry studies - General
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • NMR spectroscopy Spectrum Analysis Techniques, analytical method
  • structural analysis analytical method
  • Biochemistry and Molecular Biophysics
  • ENDOTOXINS

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