Abstract
The chemical structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide (LPS) from Acinetobacter baumannii strain ATCC 19606 was investigated by chemical analysis and NMR spectroscopy of oligosaccharides obtained after deacylation or mild acid hydrolysis. From the combined information the following carbohydrate backbones can be deduced: [GRAPHICS] where R-1 = H and R-2 = alpha-Glcp-(1 --> 2)-beta-Glcp-(1 --> 4)-beta-Glcp-(1 --> 4)-beta-Glcp-(1 as major and R-1 = Ac and R-2 = H as minor products. All monosaccharides are D-configured. Also, smaller oligosaccharide phosphates were identified that are thought to represent degradation products of the above structures.
Original language | English |
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Journal | European Journal of Biochemistry |
Volume | 269 |
Issue number | 2 |
Pages (from-to) | 422-430 |
ISSN | 0014-2956 |
DOIs | |
Publication status | Published - 2002 |
Externally published | Yes |
Keywords
- Acinetobacter
- Carbohydrate Conformation
- Carbohydrate Sequence
- Chromatography, Gel
- Mass Spectrometry
- Models, Molecular
- Molecular Sequence Data
- Nuclear Magnetic Resonance, Biomolecular
- O Antigens
- bacterium lipopolysaccharide
- Acinetobacter baumannii
- article
- carbohydrate analysis
- controlled study
- deacetylation
- degradation
- hydrolysis
- nonhuman
- nuclear magnetic resonance spectroscopy
- phosphorylation
- priority journal
- Bacteria (microorganisms)
- Negibacteria
- Core region
- Lipopolysaccharide
- NMR spectroscopy
- Structural analysis
- lipopolysaccharide
- core region
- structural analysis
- BIOCHEMISTRY
- O-ANTIGENIC POLYSACCHARIDE
- GENUS-SPECIFIC EPITOPE
- SEROLOGICAL CHARACTERIZATION
- LIPID-A
- HAEMOLYTICUS
- CHLAMYDIA
- ACID
- OLIGOSACCHARIDE
- TRACHOMATIS
- NCTC-10305
- Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Neisseriaceae [06507] Acinetobacter baumannii strain-ATCC 19606
- lipopolysaccharide carbohydrate backbone, structure
- oligosaccharides
- 10060, Biochemistry studies - General
- 10066, Biochemistry studies - Lipids
- 10068, Biochemistry studies - Carbohydrates
- 31000, Physiology and biochemistry of bacteria
- NMR spectroscopy Spectrum Analysis Techniques, analytical method
- structural analysis analytical method
- Biochemistry and Molecular Biophysics
- ENDOTOXINS