The simultaneous biosynthesis and uptake of amino acids by Lactococcus lactis studied by C-13-labeling experiments

N.B.S. Jensen, B. Christensen, Jette Nielsen, John Villadsen

    Research output: Contribution to journalJournal articleResearchpeer-review


    Uniformly C-13 labeled glucose was fed to a lactic acid bacterium growing on a defined medium supplemented with all proteinogenic amino acids except glutamate. Aspartate stemming from the protein pool and from the extracellular medium was enriched with C-13 disclosing a substantial de novo biosynthesis of this amino acid simultaneous to its uptake from the growth medium and a rapid exchange flux of aspartate over the cellular membrane. Phenylalanine, alanine, and threonine were also synthesized de novo in spite of their presence in the growth medium.
    Original languageEnglish
    JournalBiotechnology and Bioengineering
    Issue number1
    Pages (from-to)11-16
    Publication statusPublished - 2002

    Cite this