The role of the gills as potential portal of entry in rainbow trout fry syndrome

Maya Maria Mihályi Henriksen, Per Walther Kania, Lone Madsen, Kurt Buchmann, Inger Dalsgaard

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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    Abstract

    The Gram negative fish pathogen Flavobacterium psychrophilum is the causative agent of rainbow trout fry syndrome (RTFS), also known as BCWD (bacterial cold water disease). While injection-based experimental challenges with F. psychrophilum have been standardized and result in high mortality rates, the same has not been the case for immersion based models. However, injection is not a proper approach for investigations of the immune response since the first line of defense is bypassed. This study aims at understanding the immune response in the gills following infection as well as their possible role as portal of entry. A bath model, using hydrogen peroxide (H2O2) as a pre-treatment stressor to increase the number of infected fish, was used for sampling. The experimental setup consisted of four treatment groups; 1) untreated controls, 2) H2O2, 3) F. psychrophilum and 4) H2O2 and F. psychrophilum. The use of this model also provides information regarding the impact of H2O2 alone, though this was not the main objective. Samples were taken 4 hours, 2 days, 5 days and 8 days after infection and preserved for later processing. Two methods are employed in this study. Firstly, quantitative real-time PCR will be used to investigate the immune response in the gills after immersion exposure to F. psychrophilum. Secondly, fluorescent in situ hybridization will be used to visualize pathogen entry and spread in gills and head region. Samples are still undergoing analysis and results will be presented at the workshop.
    Original languageEnglish
    Title of host publicationDAFINET Workshop : Immune Responses in Fish : Book of abstracts
    Number of pages1
    PublisherUniversity of Copenhagen
    Publication date2012
    Pages19
    Publication statusPublished - 2012
    EventDAFINET Workshop : Immune Responses in Fish - University of Copenhagen, Copenhagen, Denmark
    Duration: 6 Nov 20127 Nov 2012
    http://www.dafinet.dk

    Workshop

    WorkshopDAFINET Workshop : Immune Responses in Fish
    LocationUniversity of Copenhagen
    CountryDenmark
    CityCopenhagen
    Period06/11/201207/11/2012
    Internet address

    Cite this

    Henriksen, M. M. M., Kania, P. W., Madsen, L., Buchmann, K., & Dalsgaard, I. (2012). The role of the gills as potential portal of entry in rainbow trout fry syndrome. In DAFINET Workshop : Immune Responses in Fish: Book of abstracts (pp. 19). University of Copenhagen.
    Henriksen, Maya Maria Mihályi ; Kania, Per Walther ; Madsen, Lone ; Buchmann, Kurt ; Dalsgaard, Inger. / The role of the gills as potential portal of entry in rainbow trout fry syndrome. DAFINET Workshop : Immune Responses in Fish: Book of abstracts. University of Copenhagen, 2012. pp. 19
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    abstract = "The Gram negative fish pathogen Flavobacterium psychrophilum is the causative agent of rainbow trout fry syndrome (RTFS), also known as BCWD (bacterial cold water disease). While injection-based experimental challenges with F. psychrophilum have been standardized and result in high mortality rates, the same has not been the case for immersion based models. However, injection is not a proper approach for investigations of the immune response since the first line of defense is bypassed. This study aims at understanding the immune response in the gills following infection as well as their possible role as portal of entry. A bath model, using hydrogen peroxide (H2O2) as a pre-treatment stressor to increase the number of infected fish, was used for sampling. The experimental setup consisted of four treatment groups; 1) untreated controls, 2) H2O2, 3) F. psychrophilum and 4) H2O2 and F. psychrophilum. The use of this model also provides information regarding the impact of H2O2 alone, though this was not the main objective. Samples were taken 4 hours, 2 days, 5 days and 8 days after infection and preserved for later processing. Two methods are employed in this study. Firstly, quantitative real-time PCR will be used to investigate the immune response in the gills after immersion exposure to F. psychrophilum. Secondly, fluorescent in situ hybridization will be used to visualize pathogen entry and spread in gills and head region. Samples are still undergoing analysis and results will be presented at the workshop.",
    author = "Henriksen, {Maya Maria Mih{\'a}lyi} and Kania, {Per Walther} and Lone Madsen and Kurt Buchmann and Inger Dalsgaard",
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    Henriksen, MMM, Kania, PW, Madsen, L, Buchmann, K & Dalsgaard, I 2012, The role of the gills as potential portal of entry in rainbow trout fry syndrome. in DAFINET Workshop : Immune Responses in Fish: Book of abstracts. University of Copenhagen, pp. 19, DAFINET Workshop : Immune Responses in Fish, Copenhagen, Denmark, 06/11/2012.

    The role of the gills as potential portal of entry in rainbow trout fry syndrome. / Henriksen, Maya Maria Mihályi; Kania, Per Walther ; Madsen, Lone; Buchmann, Kurt; Dalsgaard, Inger.

    DAFINET Workshop : Immune Responses in Fish: Book of abstracts. University of Copenhagen, 2012. p. 19.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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    T1 - The role of the gills as potential portal of entry in rainbow trout fry syndrome

    AU - Henriksen, Maya Maria Mihályi

    AU - Kania, Per Walther

    AU - Madsen, Lone

    AU - Buchmann, Kurt

    AU - Dalsgaard, Inger

    PY - 2012

    Y1 - 2012

    N2 - The Gram negative fish pathogen Flavobacterium psychrophilum is the causative agent of rainbow trout fry syndrome (RTFS), also known as BCWD (bacterial cold water disease). While injection-based experimental challenges with F. psychrophilum have been standardized and result in high mortality rates, the same has not been the case for immersion based models. However, injection is not a proper approach for investigations of the immune response since the first line of defense is bypassed. This study aims at understanding the immune response in the gills following infection as well as their possible role as portal of entry. A bath model, using hydrogen peroxide (H2O2) as a pre-treatment stressor to increase the number of infected fish, was used for sampling. The experimental setup consisted of four treatment groups; 1) untreated controls, 2) H2O2, 3) F. psychrophilum and 4) H2O2 and F. psychrophilum. The use of this model also provides information regarding the impact of H2O2 alone, though this was not the main objective. Samples were taken 4 hours, 2 days, 5 days and 8 days after infection and preserved for later processing. Two methods are employed in this study. Firstly, quantitative real-time PCR will be used to investigate the immune response in the gills after immersion exposure to F. psychrophilum. Secondly, fluorescent in situ hybridization will be used to visualize pathogen entry and spread in gills and head region. Samples are still undergoing analysis and results will be presented at the workshop.

    AB - The Gram negative fish pathogen Flavobacterium psychrophilum is the causative agent of rainbow trout fry syndrome (RTFS), also known as BCWD (bacterial cold water disease). While injection-based experimental challenges with F. psychrophilum have been standardized and result in high mortality rates, the same has not been the case for immersion based models. However, injection is not a proper approach for investigations of the immune response since the first line of defense is bypassed. This study aims at understanding the immune response in the gills following infection as well as their possible role as portal of entry. A bath model, using hydrogen peroxide (H2O2) as a pre-treatment stressor to increase the number of infected fish, was used for sampling. The experimental setup consisted of four treatment groups; 1) untreated controls, 2) H2O2, 3) F. psychrophilum and 4) H2O2 and F. psychrophilum. The use of this model also provides information regarding the impact of H2O2 alone, though this was not the main objective. Samples were taken 4 hours, 2 days, 5 days and 8 days after infection and preserved for later processing. Two methods are employed in this study. Firstly, quantitative real-time PCR will be used to investigate the immune response in the gills after immersion exposure to F. psychrophilum. Secondly, fluorescent in situ hybridization will be used to visualize pathogen entry and spread in gills and head region. Samples are still undergoing analysis and results will be presented at the workshop.

    M3 - Conference abstract in proceedings

    SP - 19

    BT - DAFINET Workshop : Immune Responses in Fish

    PB - University of Copenhagen

    ER -

    Henriksen MMM, Kania PW, Madsen L, Buchmann K, Dalsgaard I. The role of the gills as potential portal of entry in rainbow trout fry syndrome. In DAFINET Workshop : Immune Responses in Fish: Book of abstracts. University of Copenhagen. 2012. p. 19