Using different cell densities and microfluidic perfusion cell cultures to suppress the effects of cell-released factors, we have demonstrated the significant role played by auto- or paracrine signaling in adipocyte differentiation. The cell-released factor(s) were shown to act in the recruitment phase of the differentiation process.
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This work was supported by Grant No. 2106-08-0018 “ProCell” and grant no. 09-067112, under the Programme Commission on Strategic Growth Technologies, the Danish Agency for Science, Technology and Innovation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.