The Role of alpha-Glucosidase in Germinating Barley Grains

Duncan Stanley, Martin Rejzek, Henrik Næsted, Mark Smedley, Sofia Otero, Brendan Fahy, Frazer Thorpe, Robert J. Nash, Wendy Harwood, Birte Svensson, Kay Denyer, Robert A. Field, Alison M. Smith

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The importance of alpha-glucosidase in the endosperm starch metabolism of barley (Hordeum vulgare) seedlings is poorly understood. The enzyme converts maltose to glucose (Glc), but in vitro studies indicate that it can also attack starch granules. To discover its role in vivo, we took complementary chemical-genetic and reverse-genetic approaches. We identified iminosugar inhibitors of a recombinant form of an alpha-glucosidase previously discovered in barley endosperm (ALPHA-GLUCOSIDASE97 [HvAGL97]), and applied four of them to germinating grains. All four decreased the Glc-to-maltose ratio in the endosperm 10 d after imbibition, implying inhibition of maltase activity. Three of the four inhibitors also reduced starch degradation and seedling growth, but the fourth did not affect these parameters. Inhibition of starch degradation was apparently not due to inhibition of amylases. Inhibition of seedling growth was primarily a direct effect of the inhibitors on roots and coleoptiles rather than an indirect effect of the inhibition of endosperm metabolism. It may reflect inhibition of glycoprotein-processing glucosidases in these organs. In transgenic seedlings carrying an RNA interference silencing cassette for HvAgl97, alpha-glucosidase activity was reduced by up to 50%. There was a large decrease in the Glc-to-maltose ratio in these lines but no effect on starch degradation or seedling growth. Our results suggest that the alpha-glucosidase HvAGL97 is the major endosperm enzyme catalyzing the conversion of maltose to Glc but is not required for starch degradation. However, the effects of three glucosidase inhibitors on starch degradation in the endosperm indicate the existence of unidentified glucosidase(s) required for this process.
    Original languageEnglish
    JournalPlant Physiology
    Volume155
    Issue number2
    Pages (from-to)932-943
    ISSN0032-0889
    DOIs
    Publication statusPublished - 2011

    Bibliographical note

    Open Access articles that can be viewed online on publishers website without a subscription.
    The online version of this article on publishers website contains Web-only data.

    Fingerprint

    Dive into the research topics of 'The Role of alpha-Glucosidase in Germinating Barley Grains'. Together they form a unique fingerprint.

    Cite this