The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida

Alejandro Arce-Rodriguez, Belen Calles, Pablo Ivan Nikel, Victor de Lorenzo

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The natural physiological regime of the soil bacterium Pseudomonas putida involves incessant exposure to endogenous metabolic conflicts and environmental physicochemical insults. Yet, the role of assisted small RNA-mRNA pairing in the stress tolerance super-phenotype that is the trademark of this bacterium has not been accredited. We have thoroughly explored the physiological consequences - in particular those related to exogenous stress - of deleting the hfq gene of P. putida, which encodes the major RNA chaperone that promotes sRNA-target mRNA interactions. While the overall trend was a general weakening of every robustness descriptor of the Δhfq strain, growth parameters and production of central metabolic enzymes were comparatively less affected than other qualities that depend directly on energy status (e.g. motility, DNA repair). The overall catalytic vigour of the mutant decreased to
Original languageEnglish
JournalEnvironmental Microbiology
Volume18
Issue number10
Pages (from-to)3309-3326
Number of pages18
ISSN1462-2912
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint Dive into the research topics of 'The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida'. Together they form a unique fingerprint.

Cite this