TY - GEN
T1 - The Pseudomonas aeruginosa whole genome sequence
T2 - A 20th anniversary celebration
AU - Brinkman, Fiona S.L.
AU - Winsor, Geoffrey L.
AU - Done, Rachel E.
AU - Filloux, Alain
AU - Francis, Vanessa I.
AU - Goldberg, Joanna B.
AU - Greenberg, E. Peter
AU - Han, Kook
AU - Hancock, Robert E.W.
AU - Haney, Cara H.
AU - Häußler, Susanne
AU - Klockgether, Jens
AU - Lamont, Iain L.
AU - Levesque, Roger C.
AU - Lory, Stephen
AU - Nikel, Pablo I.
AU - Porter, Steven L.
AU - Scurlock, Matthew W.
AU - Schweizer, Herbert P.
AU - Tümmler, Burkhard
AU - Wang, Meng
AU - Welch, Martin
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021
Y1 - 2021
N2 - Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time—including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.
AB - Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time—including a large proportion of metabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.
KW - Cystic fibrosis
KW - PAO1
KW - Pseudomonas aeruginosa
KW - Whole genome sequence
U2 - 10.1016/bs.ampbs.2021.07.001
DO - 10.1016/bs.ampbs.2021.07.001
M3 - Article in proceedings
C2 - 34836612
AN - SCOPUS:85119346123
VL - 79
T3 - Advances in Microbial Physiology
SP - 25
EP - 88
BT - Advances in Microbial Physiology
PB - Klüwer Academic Press
ER -