THE PROTECTIVE MECHANISMS INDUCED BY A FISH RHABDOVIRUS DNA-VACCINE DEPENDS ON TTEMPERATURE

Niels Lorenzen, Ellen Lorenzen, Katja Einer-Jensen, Jesper Skou Rasmussen, Torben Egil Kjær, Bertrand Collet, Chris J. Secombes

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    Abstract

    DNA-vaccines encoding the viral glycoproteins of viral haemorrhagic septicaemia virus (VHSV) and infectious haematopoietic necrosis virus (IHNV) have proved highly efficient in rainbow trout (Oncorhynchus mykiss) under experimental conditions. In the early phase following vaccination, innate cross-protective mechanisms are dominating but the protection becomes highly specific within 3-4 weeks at 12-15C. Temperature is known as an important external parameter affecting the immune response in fish and the present study aimed at characterizing temperature effects on the immune response to a VHS DNA vaccine. Rainbow trout fingerlings acclimated at 5°C, 10°C or 15C, were given an intramuscular injection of 1g purified plasmid DNA and challenged with virulent VHSV 9 or 36-40 days later. The vaccine protected the fish well at all three temperatures, however the non-specific mechanisms lasted for a longer period of time at lower temperatures, hereby apparently compensating for a delayed adaptive response. At 36 dpv fish kept at 5C thus had no detectable response of neutralising antibodies while 67% of the fish kept at 15C had seroconverted. Immunohistochemical time-course studies of the injection site of vaccinated fish also showed a clear effect of temperature: in fish maintained at 15°C the vhsG-protein occurred earlier on the surface of transfected myocytes than in fish kept at the lower temperatures and the inflammatory response clearing away these myocytes similarly arose earlier at high temperature. Long persistence of transfected myocytes expressing the vaccine antigen might explain the prolonged stimulation of innate protective mechanisms at low temperature. From a practical point of view the results suggest that DNA vaccination against rhabdoviruses might be applied as a prophylactic measure within a broad temperature range.
    Original languageEnglish
    Publication date2009
    Publication statusPublished - 2009
    Event11th Congress of the International Society of Developmental and Comparative Immunology (ISDCI) - Prague, Poland
    Duration: 28 Jun 20093 Jul 2012
    Conference number: 11

    Conference

    Conference11th Congress of the International Society of Developmental and Comparative Immunology (ISDCI)
    Number11
    CountryPoland
    CityPrague
    Period28/06/200903/07/2012

    Cite this

    Lorenzen, N., Lorenzen, E., Einer-Jensen, K., Rasmussen, J. S., Kjær, T. E., Collet, B., & Secombes, C. J. (2009). THE PROTECTIVE MECHANISMS INDUCED BY A FISH RHABDOVIRUS DNA-VACCINE DEPENDS ON TTEMPERATURE. Abstract from 11th Congress of the International Society of Developmental and Comparative Immunology (ISDCI), Prague, Poland.