The production of Pseudomonas putida for the hydroxylation of toluene to its cis-glycol

CJ Hack, John Woodley, MD Lilly, J. M. Lidell

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A constitutive blocked mutant (UV4) of Pseudomonas putida was grown in a 2.5-1 fermenter on a mineral salts medium. Glucose was fed normally over an 18-h period. Gluconate reached about 5 gl(-1) in the medium and then fell to zero as it was utilised. The maximum toluene dioxygenase specific activity (2 gg(-1) h(-1)) was obtained over the last 6 h of the fermentation when the pH was fully controlled. In fermentations done at low dissolved O-2 tension (DOT) values there was an overall reduction in the cellular enzyme level. When stored at 4 degrees C in phosphate buffer, pH 7.0, harvested bacteria lost half their activity in about 90 h.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume41
Issue number5
Pages (from-to)495-499
ISSN0175-7598
Publication statusPublished - 1994
Externally publishedYes

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